Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool.

by Katherine F Donovan, Mudra Hegde, Meagan Sullender, Emma W Vaimberg, Cory M Johannessen, David E Root, John G Doench
Published 2017-01-01

Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression

by Joseph Rosenbluh, Han Xu, William Harrington, Stanley Gill, Xiaoxing Wang, Francisca Vazquez, David E. Root, Aviad Tsherniak, William C. Hahn
Published 2017-05-01

Chronos: a cell population dynamics model of CRISPR experiments that improves inference of gene fitness effects

by Joshua M. Dempster, Isabella Boyle, Francisca Vazquez, David E. Root, Jesse S. Boehm, William C. Hahn, Aviad Tsherniak, James M. McFarland
Published 2021-12-01

A Targeted RNA Interference Screen Reveals Novel Epigenetic Factors That Regulate Herpesviral Gene Expression

by Hyung Suk Oh, Kevin F. Bryant, Thomas J. F. Nieland, Aprotim Mazumder, Mukta Bagul, Mark Bathe, David E. Root, David M. Knipe
Published 2014-02-01

Morphological Profiles of RNAi-Induced Gene Knockdown Are Highly Reproducible but Dominated by Seed Effects.

by Shantanu Singh, Xiaoyun Wu, Vebjorn Ljosa, Mark-Anthony Bray, Federica Piccioni, David E Root, John G Doench, Jesse S Boehm, Anne E Carpenter
Published 2015-01-01

High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.

by Thomas J F Nieland, David J Logan, Jessica Saulnier, Daniel Lam, Caroline Johnson, David E Root, Anne E Carpenter, Bernardo L Sabatini
Published 2014-01-01

Aire-dependent genes undergo Clp1-mediated 3’UTR shortening associated with higher transcript stability in the thymus

by Clotilde Guyon, Nada Jmari, Francine Padonou, Yen-Chin Li, Olga Ucar, Noriyuki Fujikado, Fanny Coulpier, Christophe Blanchet, David E Root, Matthieu Giraud
Published 2020-04-01

Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis

by Baobing Zhao, Ganesan Keerthivasan, Yang Mei, Jing Yang, James McElherne, Piu Wong, John G. Doench, Gang Feng, David E. Root, Peng Ji
Published 2014-07-01

CRISPR-Cas9 screens reveal common essential miRNAs in human cancer cell lines

by Daniel J. Merk, Linda Paul, Foteini Tsiami, Helen Hohenthanner, Ghazal Mohseni Kouchesfahani, Lara A. Haeusser, Bianca Walter, Adam Brown, Nicole S. Persky, David E. Root, Ghazaleh Tabatabai
Published 2024-06-01

Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map.

by Ian Smith, Peyton G Greenside, Ted Natoli, David L Lahr, David Wadden, Itay Tirosh, Rajiv Narayan, David E Root, Todd R Golub, Aravind Subramanian, John G Doench
Published 2017-11-01

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

by Kendall R. Sanson, Ruth E. Hanna, Mudra Hegde, Katherine F. Donovan, Christine Strand, Meagan E. Sullender, Emma W. Vaimberg, Amy Goodale, David E. Root, Federica Piccioni, John G. Doench
Published 2018-12-01

The master regulator of the cellular stress response (HSF1) is critical for orthopoxvirus infection.

by Claire Marie Filone, Ignacio S Caballero, Ken Dower, Marc L Mendillo, Glenn S Cowley, Sandro Santagata, Daniel K Rozelle, Judy Yen, Kathleen H Rubins, Nir Hacohen, David E Root, Lisa E Hensley, John Connor
Published 2014-02-01

Partial gene suppression improves identification of cancer vulnerabilities when CRISPR-Cas9 knockout is pan-lethal

by J. Michael Krill-Burger, Joshua M. Dempster, Ashir A. Borah, Brenton R. Paolella, David E. Root, Todd R. Golub, Jesse S. Boehm, William C. Hahn, James M. McFarland, Francisca Vazquez, Aviad Tsherniak
Published 2023-08-01

CK1epsilon is required for breast cancers dependent on beta-catenin activity.

by So Young Kim, Ian F Dunn, Ron Firestein, Piyush Gupta, Leslie Wardwell, Kara Repich, Anna C Schinzel, Ben Wittner, Serena J Silver, David E Root, Jesse S Boehm, Sridhar Ramaswamy, Eric S Lander, William C Hahn
Published 2010-02-01

PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA

by Tovah A. Day, Jacob V. Layer, J. Patrick Cleary, Srijoy Guha, Kristen E. Stevenson, Trevor Tivey, Sunhee Kim, Anna C. Schinzel, Francesca Izzo, John Doench, David E. Root, William C. Hahn, Brendan D. Price, David M. Weinstock
Published 2017-04-01

Correction: Corrigendum: PARP3 is a promoter of chromosomal rearrangements and limits G4 DNA

by Tovah A. Day, Jacob V. Layer, J. Patrick Cleary, Srijoy Guha, Kristen E. Stevenson, Trevor Tivey, Sunhee Kim, Anna C Schinzel, Francesca Izzo, John Doench, David E. Root, William C. Hahn, Brendan D. Price, David M. Weinstock
Published 2017-06-01

Phenotypic Characterization of a Comprehensive Set of MAPK1/ERK2 Missense Mutants

by Lisa Brenan, Aleksandr Andreev, Ofir Cohen, Sasha Pantel, Atanas Kamburov, Davide Cacchiarelli, Nicole S. Persky, Cong Zhu, Mukta Bagul, Eva M. Goetz, Alex B. Burgin, Levi A. Garraway, Gad Getz, Tarjei S. Mikkelsen, Federica Piccioni, David E. Root, Cory M. Johannessen
Published 2016-10-01

Germline variation contributes to false negatives in CRISPR-based experiments with varying burden across ancestries

by Sean A. Misek, Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh, Isabella Boyle, Priyanka Roy, Joshua Dempster, Lia Petronio, Katherine Huang, Alham Saadat, Thomas Green, Adam Brown, John G. Doench, David E. Root, James M. McFarland, Rameen Beroukhim, Jesse S. Boehm
Published 2024-06-01

Mapping the landscape of genetic dependencies in chordoma

by Tanaz Sharifnia, Mathias J. Wawer, Amy Goodale, Yenarae Lee, Mariya Kazachkova, Joshua M. Dempster, Sandrine Muller, Joan Levy, Daniel M. Freed, Josh Sommer, Jérémie Kalfon, Francisca Vazquez, William C. Hahn, David E. Root, Paul A. Clemons, Stuart L. Schreiber
Published 2023-04-01

Regulation of Cellular Heterogeneity and Rates of Symmetric and Asymmetric Divisions in Triple-Negative Breast Cancer

by Roy Z. Granit, Hadas Masury, Reba Condiotti, Yaakov Fixler, Yael Gabai, Tzofia Glikman, Simona Dalin, Eitan Winter, Yuval Nevo, Einat Carmon, Tamar Sella, Amir Sonnenblick, Tamar Peretz, Ulrich Lehmann, Keren Paz, Federica Piccioni, Aviv Regev, David E. Root, Ittai Ben-Porath
Published 2018-09-01