The main objective of this paper is to design and implement Hybrid Controllers, which consist of Adaptive Fuzzy PID Controller (AFPIDC) and Adaptive Neuro-Fuzzy Inference System (ANFIS) for flow control application. The implementation has been accomplished onto mobile pilot plant for flow control process unit. Currently, controlling and tuning is done via KONICS PID controller that is mounted on the local control panel. However, it is unable to provide adequate response and need to be manually tuned. Thus, the AFPIDC and ANFIS are developed and implemented as alternatives to the existing PID controller with the capability of Human Machine Interface (HMI) using MATLAB/Simulink. For AFPIDC, Fuzzy Logic reasoning is used to produce adaptive PID gain while for ANFIS; Fuzzy Logic will be tuned by using Artificial Neural Network (ANN) algorithm. Overall, the control performances for PID, AFPIDC and ANFIS will be compared and analyzed for flow control application. © 2012 IFSA.