Incorporation of antimicrobial agents in concrete can enhance the resistance of the concrete to biodeterioration. However, there is lack of adequate knowledge on the effect of antimicrobial agents on performance measures of high-performance concrete (HPC), particularly during early strength development, when the hydration reaction is ongoing, is not well understood. Therefore, the effect of antimicrobial agents on early compressive strength (CS) of HPC was investigated. The cement in HPC samples was partially replaced with nanosized titanium dioxide (TiO2) and zinc oxide (ZnO) by up to 2 by weight of the cement. Using response surface methodology, 21 combinations of TiO2 and ZnO contents were generated. The combinations were adopted for preparation of the samples. Individual and combined effects of TiO2 and ZnO on 7-day CS of the samples were evaluated. The standard form of the second-order response surface model is adopted to develop a model to fit the strength data. Statistics of the data verify that the model can be employed to predict the 7-day CS of HPC that employs nanosized TiO2 and ZnO as antimicrobial agents. © 2024 EDP Sciences. All rights reserved.