Conventionally, increasing the yield of microalgal biomass has been the primary focus of research, while the significant protein reserve within this biomass has remained largely unexplored. This protein reserve possesses substantial value and versatility, offering a wide range of prospective applications and presenting an enticing chance for innovation and value enhancement for various sectors. Current study employed an innovative research approach that focused solely on the LCA of protein production potential from microalgal biomass, a lesser-explored aspects within this domain. Most environmental impact categories were shown to be significantly affected by cultivation phase because of the electrical obligation, followed by the harvesting and protein extraction phase. Still, the environmental aspect was seen to yield a minimal impact on global warming potential, i.e., 4 � 10�3 kg CO2, underscoring the ecologically favorable nature of the process. Conversely, the overall energy impact was seen to intensify with NEB of � 39.33 MJ and NER of 0.49, drawing attention to the importance of addressing the energy aspect to harness the full potential of microalgal protein production. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.