Membrane fouling significantly hinders the widespread application of membrane technology. In the current study, a support vector machine (SVM) and artificial neural networks (ANN) modelling approach was adopted to optimize the membrane permeability in a novel membrane rotating biological contactor (MRBC). The MRBC utilizes the disk rotation mechanism to generate a shear rate at the membrane surface to scour off the foulants. The effect of operational parameters (disk rotational speed, hydraulic retention time (HRT), and sludge retention time (SRT)) was studied on the membrane permeability. ANN and SVM are machine learning algorithms that aim to predict the model based on the trained data sets. The implementation and efficacy of machine learning and statistical approaches have been demonstrated through real-time experimental results. Feed-forward ANN with the back-propagation algorithm and SVN regression models for various kernel functions were trained to augment the membrane permeability. An overall comparison of predictive models for the test data sets reveals the model�s significance. ANN modelling with 13 hidden layers gives the highest R2 value of >0.99, and the SVM model with the Bayesian optimizer approach results in R2 values higher than 0.99. The MRBC is a promising substitute for traditional suspended growth processes, which aligns with the stipulations of ecological evolution and environmentally friendly treatment. © 2022 by the authors.