Graphene oxide (GO) is a novel class of 2D nanoscale material with remarkable properties such as excellent hydrophilic nature, higher aspect ratio and larger internal strength which results in the overall improvement of nanocomposite performance once uniform dispersion is achieved. Ultrasonication (tip based) has emerged as an efficient approach for uniform dispersion of GO solution. This study aims to identify an appropriate range of GO concentration which is of higher quality, higher aspect ratio and lower structural defects for performance enhancement of cement composites. Raman spectroscopy technique has been adopted to find an appropriate range of GO concentration (0.01, 0.0275, 0.045, 0.0625, 0.0963 and 0.125 mg/mL) on the basis of defect ratio evolved under the effect of tip sonication parameter. Results imply that the defect ratio is higher for 0.01 GO concentration as compared to others. Furthermore, an ultrasonication duration of 10 min for GO solution produces better results as quantified by Raman analysis. © 2021, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.