Human papilloma virus DNA-biomarker analysis for cervical cancer: Signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy

Lv, Q. and Wang, Y. and Su, C. and Lakshmipriya, T. and Gopinath, S.C.B. and Pandian, K. and Perumal, V. and Liu, Y. (2019) Human papilloma virus DNA-biomarker analysis for cervical cancer: Signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy. International Journal of Biological Macromolecules, 134. pp. 354-360. ISSN 01418130

Full text not available from this repository.
Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Human papillomavirus (HPV) is a double-standard DNA virus, as well as the source of infection to the mucous membrane. It is a sexually transmitted disease that brings the changes in the cervix cells. Oncogenes, E6 and E7 play a pivotal role in the HPV infection. Identifying these genes to detect HPV strains, especially a prevalent HPV16 strain, will bring a great impact. Among different sensing strategies for pathogens, the dielectric electrochemical biosensor shows the potential due to its higher sensitivity. In this research, HPV16-E7 DNA sequence was detected on the carbodiimidazole-modified interdigitated electrode (IDE) surface with the detection limit of 1 fM. To enhance the sensitivity, the target sequence was conjugated on gold nanoparticle (GNP) and attained detection to the level of 10 aM. This produced ~100 folds improvement in detecting HPV16-E7 gene and 4 folds increment in the current flow. The stability of HPV16-E7 DNA sequences on GNP was verified by the salt-induced GNP aggregation. The current system has shown the higher specificity by comparing against non-complementary and triple-mismatched DNA sequences of HPV16-E7. This demonstration in detecting HPV16-E7 using dielectric IDE sensing system with a higher sensitivity can be recommended for detecting a wide range of disease-causing DNA-markers. © 2019

Item Type: Article
Additional Information: cited By 46
Uncontrolled Keywords: biotin; gold nanoparticle; protein E7; sodium chloride; streptavidin; tumor marker; virus DNA; biological marker; biotin; gold; metal nanoparticle; oncogene protein E7, Human papillomavirus type 16; protein E7; streptavidin; virus DNA, Article; controlled study; DNA sequence; Human papillomavirus type 16; limit of detection; nonhuman; uterine cervix cancer; Wart virus; chemistry; female; genetic procedures; genetics; human; metabolism; nucleotide sequence; procedures; uterine cervix tumor; virology, Base Sequence; Biomarkers; Biosensing Techniques; Biotin; DNA, Viral; Female; Gold; Humans; Limit of Detection; Metal Nanoparticles; Papillomavirus E7 Proteins; Streptavidin; Uterine Cervical Neoplasms
Depositing User: Mr Ahmad Suhairi UTP
Date Deposited: 10 Nov 2023 03:25
Last Modified: 10 Nov 2023 03:25
URI: https://khub.utp.edu.my/scholars/id/eprint/11439

Actions (login required)

View Item
View Item