From nuclease-based gene knock-in to prime editing – promising technologies of precision gene engineering

by Tetsushi Sakuma
Published 2022-12-01

Genome editing

by Maura McGrail, Tetsushi Sakuma, Leonidas Bleris
Published 2022-11-01

Establishment of CRFK cells for vaccine production by inactivating endogenous retrovirus with TALEN technology

by Sayumi Shimode, Tetsushi Sakuma, Takashi Yamamoto, Takayuki Miyazawa
Published 2022-04-01

Establishment of pten knockout medaka with transcription activator-like effector nucleases (TALENs) as a model of PTEN deficiency disease.

by Yuriko Matsuzaki, Tetsushi Sakuma, Takashi Yamamoto, Hideyuki Saya
Published 2017-01-01

Genome editing for biodiesel production in oleaginous microalga, Nannochloropsis species

by Tomokazu Kurita, Masako Iwai, Hiroyuki Ohta, Tetsushi Sakuma, Takashi Yamamoto
Published 2023-12-01

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

by Tetsushi Sakuma, Mitsumasa Takenaga, Yoshinori Kawabe, Takahiro Nakamura, Masamichi Kamihira, Takashi Yamamoto
Published 2015-10-01

Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference.

by Yusuke Sato'o, Junzo Hisatsune, Liansheng Yu, Tetsushi Sakuma, Takashi Yamamoto, Motoyuki Sugai
Published 2018-01-01

Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice

by Yoshiko Nakagawa, Tetsushi Sakuma, Norihisa Nishimichi, Yasuyuki Yokosaki, Toru Takeo, Naomi Nakagata, Takashi Yamamoto
Published 2017-05-01

PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells

by Yusuke Kaku, Atsuhiro Taguchi, Shunsuke Tanigawa, Fahim Haque, Tetsushi Sakuma, Takashi Yamamoto, Ryuichi Nishinakamura
Published 2017-07-01

A high excision potential of TALENs for integrated DNA of HIV-based lentiviral vector.

by Hirotaka Ebina, Yuka Kanemura, Naoko Misawa, Tetsushi Sakuma, Tomoko Kobayashi, Takashi Yamamoto, Yoshio Koyanagi
Published 2015-01-01

Robust protein-based engineering of hepatocyte-like cells from human mesenchymal stem cells

by Tomoki Takashina, Akihiro Matsunaga, Yukiko Shimizu, Tetsushi Sakuma, Tadashi Okamura, Kunie Matsuoka, Takashi Yamamoto, Yukihito Ishizaka
Published 2023-03-01

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice

by Yoshiko Nakagawa, Tetsushi Sakuma, Norihisa Nishimichi, Yasuyuki Yokosaki, Noriyuki Yanaka, Toru Takeo, Naomi Nakagata, Takashi Yamamoto
Published 2016-08-01

MET Activation by a Macrocyclic Peptide Agonist that Couples to Biological Responses Differently from HGF in a Context-Dependent Manner

by Wenyu Miao, Katsuya Sakai, Ryu Imamura, Kenichiro Ito, Hiroaki Suga, Tetsushi Sakuma, Takashi Yamamoto, Kunio Matsumoto
Published 2018-10-01

Activin Is Superior to BMP7 for Efficient Maintenance of Human iPSC-Derived Nephron Progenitors

by Shunsuke Tanigawa, Hidekazu Naganuma, Yusuke Kaku, Takumi Era, Tetsushi Sakuma, Takashi Yamamoto, Atsuhiro Taguchi, Ryuichi Nishinakamura
Published 2019-08-01

Biased genome editing using the local accumulation of DSB repair molecules system

by Shota Nakade, Keiji Mochida, Atsushi Kunii, Kazuki Nakamae, Tomomi Aida, Kohichi Tanaka, Naoaki Sakamoto, Tetsushi Sakuma, Takashi Yamamoto
Published 2018-08-01

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

by Takuya Sato, Tetsushi Sakuma, Tetsuhiro Yokonishi, Kumiko Katagiri, Satoshi Kamimura, Narumi Ogonuki, Atsuo Ogura, Takashi Yamamoto, Takehiko Ogawa
Published 2015-07-01

TALEN-Mediated Gene Editing of <i>slc24a5</i> (Solute Carrier Family 24, Member 5) in Kawakawa, <i>Euthynnus affinis</i>

by Dipak Pandey, Takahiro Matsubara, Taiju Saito, Yukinori Kazeto, Koichiro Gen, Tetsushi Sakuma, Takashi Yamamoto, Miyuki Mekuchi, Rie Goto
Published 2021-12-01

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

by Ken-ichi T. Suzuki, Yukiko Isoyama, Keiko Kashiwagi, Tetsushi Sakuma, Hiroshi Ochiai, Naoaki Sakamoto, Nobuaki Furuno, Akihiko Kashiwagi, Takashi Yamamoto
Published 2013-03-01

The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation

by Tatsuo Miyamoto, Kosuke Hosoba, Hiroshi Ochiai, Ekaterina Royba, Hideki Izumi, Tetsushi Sakuma, Takashi Yamamoto, Brian David Dynlacht, Shinya Matsuura
Published 2015-02-01

Generation of D1-1 TALEN isogenic control cell line from Dravet syndrome patient iPSCs using TALEN-mediated editing of the SCN1A gene

by Yasuyoshi Tanaka, Takefumi Sone, Norimichi Higurashi, Tetsushi Sakuma, Sadafumi Suzuki, Mitsuru Ishikawa, Takashi Yamamoto, Jun Mitsui, Hitomi Tsuji, Hideyuki Okano, Shinichi Hirose
Published 2018-04-01