%I American Institute of Physics Inc. %A W.N.A. Kadir %A M.K. Lam %A Y. Uemura %A J.-W. Lim %A K.T. Lee %V 2016 %T Harvesting and pre-treatment of microalgae biomass via ozonation for lipid extraction: A preliminary study %R 10.1063/1.5055466 %D 2018 %L scholars9934 %J AIP Conference Proceedings %O cited By 2; Conference of 3rd International Conference on Applied Science and Technology, ICAST 2018 ; Conference Date: 10 April 2018 Through 12 April 2018; Conference Code:140017 %X Extraction of lipid is an important step in the production of microalgae biodiesel, but its efficiency depends on the species and also the pre-treatment methods for cell disruption before undergoing extraction stage. Thus, ozonation pre-treatment were used in this study to address the knowledge gap surrounding the harvesting and pre-treatment of microalgae for efficient lipid extraction. Chlorella vulgaris was ozonized in batches using lab-scale ozone generator (HATLEA) in the range of 5 to 25 minutes where the sedimentation efficiency of microalgae was found to be optimum at 25 minutes of ozonation time and flow rate of 3 L/min. Based on these conditions, the sedimentation efficiency was 31 higher compared to non-ozonated microalgae. Besides, lipid yield was increased from 18 (non-ozonated) to 25.7 after ozonation under optimum conditions. Therefore, it can be concluded that the ozonation pre-treatment of microalgae can be applied in the overall process of microalgae biodiesel production as alternative steps to reduce the cost of production. © 2018 Author(s).