@inproceedings{scholars9934, publisher = {American Institute of Physics Inc.}, journal = {AIP Conference Proceedings}, title = {Harvesting and pre-treatment of microalgae biomass via ozonation for lipid extraction: A preliminary study}, note = {cited By 2; Conference of 3rd International Conference on Applied Science and Technology, ICAST 2018 ; Conference Date: 10 April 2018 Through 12 April 2018; Conference Code:140017}, volume = {2016}, year = {2018}, doi = {10.1063/1.5055466}, isbn = {9780735417342}, author = {Kadir, W. N. A. and Lam, M. K. and Uemura, Y. and Lim, J.-W. and Lee, K. T.}, issn = {0094243X}, abstract = {Extraction of lipid is an important step in the production of microalgae biodiesel, but its efficiency depends on the species and also the pre-treatment methods for cell disruption before undergoing extraction stage. Thus, ozonation pre-treatment were used in this study to address the knowledge gap surrounding the harvesting and pre-treatment of microalgae for efficient lipid extraction. Chlorella vulgaris was ozonized in batches using lab-scale ozone generator (HATLEA) in the range of 5 to 25 minutes where the sedimentation efficiency of microalgae was found to be optimum at 25 minutes of ozonation time and flow rate of 3 L/min. Based on these conditions, the sedimentation efficiency was 31 higher compared to non-ozonated microalgae. Besides, lipid yield was increased from 18 (non-ozonated) to 25.7 after ozonation under optimum conditions. Therefore, it can be concluded that the ozonation pre-treatment of microalgae can be applied in the overall process of microalgae biodiesel production as alternative steps to reduce the cost of production. {\^A}{\copyright} 2018 Author(s).}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054531502&doi=10.1063\%2f1.5055466&partnerID=40&md5=cbb6fb953abae2dd5fd31617e88e92ee} }