eprintid: 9417
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/00/94/17
datestamp: 2023-11-09 16:21:24
lastmod: 2023-11-09 16:21:24
status_changed: 2023-11-09 16:15:04
type: article
metadata_visibility: show
creators_name: Cheen, O.C.
creators_name: Gopinath, S.C.B.
creators_name: Perumal, V.
creators_name: Arshad, M.K.M.
creators_name: Lakshmipriya, T.
creators_name: Chen, Y.
creators_name: Haarindraprasad, R.
creators_name: Rao, B.S.
creators_name: Hashim, U.
creators_name: Pandian, K.
title: Aptamer-based impedimetric determination of the human blood clotting factor IX in serum using an interdigitated electrode modified with a ZnO nanolayer
ispublished: pub
note: cited By 28
abstract: This article describes a sensitive impedimetric method for the determination of human blood coagulation factor IX protein (FIX) which is present in extremely low concentration in serum. An interdigitated electrode (IDE) whose surface was layered with zinc oxide was modified with two kinds of probes. One is an antibody, the other an aptamer against FIX. A comparative study between anti-FIX aptamer and anti-FIX antibody showed the aptamer to possess higher affinity for FIX. A sandwich aptamer assay was worked out by using the FIX-binding aptamer on the surface of the IDE. It has a detection limit as low as 10 pM which makes it 4 to 30-fold more sensitive than any other method reported for FIX. Moreover, to practice detection in clinical samples, FIX was detected from the human blood serum by spiking. In our perception, the sensitivity of the ZnO-modified IDE presented here makes it a promising tool for sensing clinically relevant analytes that are present in very low (sub-pM) concentrations. Figure not available: see fulltext. © 2016, Springer-Verlag Wien.
date: 2017
publisher: Springer-Verlag Wien
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994210587&doi=10.1007%2fs00604-016-2001-6&partnerID=40&md5=b97c3806a1c010ecc29e67e22da1065c
id_number: 10.1007/s00604-016-2001-6
full_text_status: none
publication: Microchimica Acta
volume: 184
number: 1
pagerange: 117-125
refereed: TRUE
issn: 00263672
citation:   Cheen, O.C. and Gopinath, S.C.B. and Perumal, V. and Arshad, M.K.M. and Lakshmipriya, T. and Chen, Y. and Haarindraprasad, R. and Rao, B.S. and Hashim, U. and Pandian, K.  (2017) Aptamer-based impedimetric determination of the human blood clotting factor IX in serum using an interdigitated electrode modified with a ZnO nanolayer.  Microchimica Acta, 184 (1).  pp. 117-125.  ISSN 00263672