eprintid: 9170
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/00/91/70
datestamp: 2023-11-09 16:21:08
lastmod: 2023-11-09 16:21:08
status_changed: 2023-11-09 16:14:26
type: article
metadata_visibility: show
creators_name: Satar, I.
creators_name: Daud, W.R.W.
creators_name: Kim, B.H.
creators_name: Somalu, M.R.
creators_name: Ghasemi, M.
title: Immobilized mixed-culture reactor (IMcR) for hydrogen and methane production from glucose
ispublished: pub
keywords: Cell culture; Cells; Chemical oxygen demand; Chromatography; Cytology; Fermentation; Gas chromatography; Glucose; High performance liquid chromatography; Hydrogen; Liquid chromatography; Methane; Scanning electron microscopy; Substrates; Thermal conductivity; Thermal conductivity of gases, Fermentation time; Glucose concentration; Immobilized cell technology; Methane production; Mixed cultures; Optimal conditions; Production of hydrogen; Thermal conductivity detectors, Hydrogen production, biogas; bioreactor; chemical oxygen demand; concentration (composition); fermentation; glucose; hydrogen; methane; microorganism; temperature effect; thermal conductivity
note: cited By 18
abstract: Immobilized cell technology is a new technique to produce biogas. In the present study, an immobilized mixed-culture reactor (IMcR) in batch-mode operation was used for the production of hydrogen and methane simultaneously from glucose. Several factors, such as glucose concentration, temperature and fermentation time, were evaluated to determine the optimal conditions for hydrogen and methane production. Gas chromatography with a thermal conductivity detector (GC-TCD) and high-performance liquid chromatography (HPLC) were used to analyse the gas and effluent. The morphologies of the immobilized cells were characterized using scanning electron microscopy (SEM). The optimal conditions for hydrogen and methane production were obtained using a substrate with 5.0 g/L glucose at 60 °C for fermentation times of 48.0 h (hydrogen) and 72.0 h (methane). The maximum yields of hydrogen and methane at these optimal conditions were 37.0 ± 0.0 (�10�3) mol/mol glu and 39.0 ± 0.0 (�10�3) mol/mol glu, respectively. The chemical oxygen demand (COD) and pH gradually decreased with increasing fermentation time and temperature. However, the performance of the IMcR decreased over time due to cell damage and microorganism detachment from the cell. In conclusion, the IMcR system is a potential system for the simultaneous production of hydrogen and methane. © 2017 Elsevier Ltd
date: 2017
publisher: Elsevier Ltd
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030120001&doi=10.1016%2fj.energy.2017.08.071&partnerID=40&md5=4f4c1a043b8a76217570ab87922cf3ff
id_number: 10.1016/j.energy.2017.08.071
full_text_status: none
publication: Energy
volume: 139
pagerange: 1188-1196
refereed: TRUE
issn: 03605442
citation:   Satar, I. and Daud, W.R.W. and Kim, B.H. and Somalu, M.R. and Ghasemi, M.  (2017) Immobilized mixed-culture reactor (IMcR) for hydrogen and methane production from glucose.  Energy, 139.  pp. 1188-1196.  ISSN 03605442