eprintid: 8179
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/00/81/79
datestamp: 2023-11-09 16:20:02
lastmod: 2023-11-09 16:20:02
status_changed: 2023-11-09 16:11:59
type: article
metadata_visibility: show
creators_name: Lawrence, G.D.
creators_name: Rahmat, R.
creators_name: Makahleh, A.
creators_name: Saad, B.
title: Liquid Chromatographic Analysis of α-Dicarbonyls Using Girard-T Reagent Derivatives
ispublished: pub
keywords: 3-deoxyglucosone; 5-hydroxymethylfurfural; betaine; corn syrup; deoxyglucose; furfural; Girard's reagent T; glyoxal, analogs and derivatives; chemistry; limit of detection; liquid chromatography; procedures; reproducibility; statistical model, Betaine; Chromatography, Liquid; Deoxyglucose; Furaldehyde; Glyoxal; High Fructose Corn Syrup; Limit of Detection; Linear Models; Reproducibility of Results
note: cited By 1
abstract: The measurement of a-dicarbonyls and other degradation products of sugars has become important in view of their toxicity. Although there are several methods used for their analysis, most require long reaction times to form UV absorbing or fluorescent derivatives and the nonpolar nature of commonly used derivatives necessitates relatively high concentrations of organic solvents for elution in reverse phase liquid chromatography. The present method describes the use of Girard-T reagent in a simple, one step derivatization of a-dicarbonyls and conjugated aldehydes and analysis using ion-pair reverse phase liquid chromatography. The limit of detection was in the range of 0.06-0.09 μM (4-12 ng/mL) for glyoxal, methylglyoxal, 3-deoxyglucosone and 5-hydroxymethylfurfural with good linear response and reproducibility using UV detection. The hydrazone derivatives were stable for several days in solution. The method was used to study degradation of several sugars and quantification of the target a-dicarbonyls and 5-hydroxymethylfurfural in several soft drinks. © The Author 2017.
date: 2017
publisher: Oxford University Press
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85034852353&doi=10.1093%2fchromsci%2fbmx073&partnerID=40&md5=451b2029235d35e23d671b3fdc736d55
id_number: 10.1093/chromsci/bmx073
full_text_status: none
publication: Journal of Chromatographic Science
volume: 55
number: 10
pagerange: 1043-1050
refereed: TRUE
issn: 00219665
citation:   Lawrence, G.D. and Rahmat, R. and Makahleh, A. and Saad, B.  (2017) Liquid Chromatographic Analysis of α-Dicarbonyls Using Girard-T Reagent Derivatives.  Journal of Chromatographic Science, 55 (10).  pp. 1043-1050.  ISSN 00219665