relation: https://khub.utp.edu.my/scholars/8127/
title: Evaluation of extraction methods for the identification of proteins from date palm (Phoenix dactylifera L.) seed and flesh
creator: Lee, H.X.
creator: Ahmad, F.
creator: Saad, B.
creator: Ismail, M.N.
description: Date fruits are well known to be very nutritious. Nevertheless, the protein contents of the fruit, particularly the seed and flesh, are still understudied, largely due to their difficult physical characteristics. This study was conducted to compare three different protein extraction methods which were the trichloroacetic acid (TCA)-acetone (TCA-A), phenol (Phe), and TCA-acetone-phenol (TCA-A-Phe), and to perform proteomic analysis on date palm seed and flesh. Phe extraction method showed the highest protein yields for both seed (8.26 mg/g) and flesh (1.57 mg/g). Through sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Phe, and TCA-A-Phe extraction methods were shown to be efficient in removing interfering compounds and gave well-resolved bands over a wide range of molecular weights. Following liquid chromatography�tandem mass spectrometry analysis, about 50�64 of extracted proteins were identified with known functions including those involved in glycolysis, Krebs cycle, defense, and storage. Phe protein extraction method was proven to be the optimal method for date flesh and seed. © 2017 Taylor & Francis.
publisher: Taylor and Francis Inc.
date: 2017
type: Article
type: PeerReviewed
identifier:   Lee, H.X. and Ahmad, F. and Saad, B. and Ismail, M.N.  (2017) Evaluation of extraction methods for the identification of proteins from date palm (Phoenix dactylifera L.) seed and flesh.  Preparative Biochemistry and Biotechnology, 47 (10).  pp. 998-1007.  ISSN 10826068     
relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032837492&doi=10.1080%2f10826068.2017.1365250&partnerID=40&md5=3cbf3b55e1ad1943763b83e64d054852
relation: 10.1080/10826068.2017.1365250
identifier: 10.1080/10826068.2017.1365250