eprintid: 7120 rev_number: 2 eprint_status: archive userid: 1 dir: disk0/00/00/71/20 datestamp: 2023-11-09 16:18:55 lastmod: 2023-11-09 16:18:55 status_changed: 2023-11-09 16:08:31 type: article metadata_visibility: show creators_name: Muhammad, N. creators_name: Gao, Y. creators_name: Iqbal, F. creators_name: Ahmad, P. creators_name: Ge, R. creators_name: Nishan, U. creators_name: Rahim, A. creators_name: Gonfa, G. creators_name: Ullah, Z. title: Extraction of biocompatible hydroxyapatite from fish scales using novel approach of ionic liquid pretreatment ispublished: pub keywords: Biocompatibility; Cells; Characterization; Cytology; Fish; Fourier transform infrared spectroscopy; Gravimetric analysis; Hydroxyapatite; Ionic liquids; Liquids; Thermogravimetric analysis; X ray diffraction, Brunauer emmett tellers; Energy dispersive x-ray; Field emission scanning; Fish scale; Human embryonic kidney 293 cells; Ionic liquid pretreatment; Thermal degradation temperatures; Thermal gravimetric analyses (TGA), Extraction note: cited By 85 abstract: In this study the waste fish scales (FS) were dissolved in 1-butyl-3-methylimidazolium acetate ionic liquid to obtain a valuable product of hydroxyapatite (HAp). The HAp was obtained in the yield of 32 ± 2. The obtained HAp was characterized using Fourier Transform Infrared Spectroscopy (FTIR), Powder X-rays Diffraction (PXRD), Thermal Gravimetric Analysis (TGA), Field Emission Scanning Microscopy (FE-SEM), Energy Dispersive X-rays spectroscopy (EDX), and Brunauer-Emmett-Teller (BET). The results of FTIR and XRD showed the characteristic peaks of the HAp. The thermal degradation temperature of the extracted HAp was relatively high. Furthermore, low weight loss was measured which confirmed the removal of organic part of FS during ionic liquid treatment. The FE-SEM result showed the particles with different morphologies and EDX analysis showed a Ca/P ratio of 1.60 for the extracted HAp. The biocompatibility of the extracted HAp was assessed through MTT cell viability assay using known Human Embryonic Kidney 293 cells (HEK cells) and epidermoid carcinoma cells (A431 cells). © 2016 Elsevier B.V. All rights reserved. date: 2016 publisher: Elsevier official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956934336&doi=10.1016%2fj.seppur.2016.01.047&partnerID=40&md5=c4d0bbcf962f056f2cc466589bebd17e id_number: 10.1016/j.seppur.2016.01.047 full_text_status: none publication: Separation and Purification Technology volume: 161 pagerange: 129-135 refereed: TRUE issn: 13835866 citation: Muhammad, N. and Gao, Y. and Iqbal, F. and Ahmad, P. and Ge, R. and Nishan, U. and Rahim, A. and Gonfa, G. and Ullah, Z. (2016) Extraction of biocompatible hydroxyapatite from fish scales using novel approach of ionic liquid pretreatment. Separation and Purification Technology, 161. pp. 129-135. ISSN 13835866