eprintid: 7037 rev_number: 2 eprint_status: archive userid: 1 dir: disk0/00/00/70/37 datestamp: 2023-11-09 16:18:50 lastmod: 2023-11-09 16:18:50 status_changed: 2023-11-09 16:08:19 type: article metadata_visibility: show creators_name: Elgharbawy, A.A. creators_name: Alam, M.Z. creators_name: Moniruzzaman, M. creators_name: Goto, M. title: Ionic liquid pretreatment as emerging approaches for enhanced enzymatic hydrolysis of lignocellulosic biomass ispublished: pub keywords: Biomass; Biomaterials; Cellulose; Enzyme activity; Enzymes; Fermentation; Hydrolysis; Ionic liquids; Liquids; Saccharification, Biological properties; Biomass pre treatments; Cellulase; Cellulosic material; Ionic liquid (ils); Ionic liquid pretreatment; Lignocellulosic biomass; Single-step process, Enzymatic hydrolysis, bioethanol; biofuel; hemicellulose; hydrolase; ionic liquid; lignin; lignocellulose; polysaccharide, bioenergy; biomass; bioreactor; energy consumption; enzymatic degradation; enzyme activity; enzyme inactivation; enzyme kinetics; fermentation; hydrolysis; lignocellulosic biomass; melting point; priority journal; Review; saccharification; surface area; water content note: cited By 266 abstract: Ionic liquids (ILs) have been increasingly exploited as solvents and/or reagents in many applications due to their "green" properties as well as their tunable physicochemical and biological properties. One of them is the pretreatment of lignocellulosic biomass prior to enzymatic hydrolysis for bioenergy and biomaterials production. Generally, the process composed of an IL pretreatment/recovered followed by enzymatic hydrolysis of lignocellulosic biomass. Another approach was developed in which simultaneous pretreatment and saccharification of biomass in ILs were performed. However, the use of ILs in this integrated process, in which enzymatic hydrolysis is done in the presence of IL applied for biomass pretreatment, can easily inactivate the enzymes. Cellulases, one of the most important hydrolytic enzymes used to catalyze the polysaccharide, showed good levels of stability in many ILs. In addition, various approaches were made including synthesis of enzyme-compatible ILs, screening ILs-tolerant enzymes and media engineering to improve cellulases performance. In this review paper, recent advances of the hydrolysis of lignocellulosic biomass in a single-step process in ILs will be highlighted. Various cellulase stabilization approaches and the design of enzyme compatible biomass-dissolving ILs are also discussed. We strongly believe that IL-compatible cellulase systems would eliminate the need to recover the regenerated biomass and lead to a simple, in situ saccharification of cellulosic materials, which would be beneficial in developing integrated bioprocesses. © 2016 Elsevier B.V. date: 2016 publisher: Elsevier official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957551144&doi=10.1016%2fj.bej.2016.01.021&partnerID=40&md5=4ce32a7de2c2e3ccf08988fc6624bbf9 id_number: 10.1016/j.bej.2016.01.021 full_text_status: none publication: Biochemical Engineering Journal volume: 109 pagerange: 252-267 refereed: TRUE issn: 1369703X citation: Elgharbawy, A.A. and Alam, M.Z. and Moniruzzaman, M. and Goto, M. (2016) Ionic liquid pretreatment as emerging approaches for enhanced enzymatic hydrolysis of lignocellulosic biomass. Biochemical Engineering Journal, 109. pp. 252-267. ISSN 1369703X