eprintid: 6896
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/00/68/96
datestamp: 2023-11-09 16:18:42
lastmod: 2023-11-09 16:18:42
status_changed: 2023-11-09 16:07:57
type: article
metadata_visibility: show
creators_name: Sivapragasam, M.
creators_name: Moniruzzaman, M.
creators_name: Goto, M.
title: Recent advances in exploiting ionic liquids for biomolecules: Solubility, stability and applications
ispublished: pub
keywords: Chemical stability; DNA; Enzyme activity; Enzymes; Ionic liquids; Liquids; Microorganisms; Molecular biology; Proteins; Solubility; Solvents; Stability; Stabilization, Biological science; DNA stability; Enzymatic reaction; Ionic liquid (ils); Protein stability; Selective process; Technological development; Technological utility, Biomolecules, DNA; ionic liquid; organic solvent; reagent; water, biocatalysis; biocompatibility; biotechnology; biotransformation; enzyme activity; enzyme mechanism; priority journal; protein stability; Review; solubility; solubilization
note: cited By 145
abstract: The technological utility of biomolecules (e.g. proteins, enzymes and DNA) can be significantly enhanced by combining them with ionic liquids (ILs) � potentially attractive �green� and �designer� solvents � rather than using in conventional organic solvents or water. In recent years, ILs have been used as solvents, cosolvents, and reagents for biocatalysis, biotransformation, protein preservation and stabilization, DNA solubilization and stabilization, and other biomolecule-based applications. Using ILs can dramatically enhance the structural and chemical stability of proteins, DNA, and enzymes. This article reviews the recent technological developments of ILs in protein-, enzyme-, and DNA-based applications. We discuss the different routes to increase biomolecule stability and activity in ILs, and the design of biomolecule-friendly ILs that can dissolve biomolecules with minimum alteration to their structure. This information will be helpful to design IL-based processes in biotechnology and the biological sciences that can serve as novel and selective processes for enzymatic reactions, protein and DNA stability, and other biomolecule-based applications. Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
date: 2016
publisher: Wiley-VCH Verlag
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979768773&doi=10.1002%2fbiot.201500603&partnerID=40&md5=7f1f8e2d653819663be5bbb9d0d03e44
id_number: 10.1002/biot.201500603
full_text_status: none
publication: Biotechnology Journal
volume: 11
number: 8
pagerange: 1000-1013
refereed: TRUE
issn: 18606768
citation:   Sivapragasam, M. and Moniruzzaman, M. and Goto, M.  (2016) Recent advances in exploiting ionic liquids for biomolecules: Solubility, stability and applications.  Biotechnology Journal, 11 (8).  pp. 1000-1013.  ISSN 18606768