%0 Journal Article
%@ 20402503
%A Araki, S.
%A Wakabayashi, R.
%A Moniruzzaman, M.
%A Kamiya, N.
%A Goto, M.
%D 2015
%F scholars:6211
%I Royal Society of Chemistry
%J MedChemComm
%K 1 dodecyl 3 methyl imidazolium bis(trifluoromethyl sulfonyl)amide; immunoglobulin G; ionic liquid; ovalbumin; penetration enhancing agent; surfactant; unclassified drug, animal experiment; animal tissue; Article; controlled study; dispersion; drug delivery system; drug formulation; female; immunization; immunoglobulin blood level; in vitro study; in vivo study; mouse; nonhuman; particle size; priority journal; skin permeability; solid in oil nanodispersion
%N 12
%P 2124-2128
%R 10.1039/c5md00378d
%T Ionic liquid-mediated transcutaneous protein delivery with solid-in-oil nanodispersions
%U https://khub.utp.edu.my/scholars/6211/
%V 6
%X As a potentially safe and non-invasive vaccination method, transcutaneous immunization represents an attractive alternative to conventional vaccine delivery by injection. However, the development of transcutaneous immunization has remained a challenge for a large number of hydrophilic macromolecules including protein and peptide antigens. We report a novel ionic liquid (IL)-mediated transcutaneous vaccine formulation consisting of a solid-in-oil (S/O) nanodispersion of antigen coated with pharmaceutically accepted surfactants dispersed in IL-containing oil. The introduction of the IL C12mimTf2N (1-dodecyl-3-methyl imidazolium bis(trifluoromethyl sulfonyl) amide) as a penetration enhancer in the formulation significantly enhanced the skin permeability of ovalbumin (OVA), a model antigen. It was also found that the IL-mediated S/O nanodispersion obtained high levels of OVA-specific serum IgG compared with both S/O nanodispersions without IL and PBS control. These findings clearly indicate that ILs-which are potentially attractive "green" and "designer" solvents-could serve as potential skin penetration enhancers in transcutaneous vaccination for hydrophilic macromolecules. © 2015 The Royal Society of Chemistry.
%Z cited By 45