@article{scholars6211,
           pages = {2124--2128},
       publisher = {Royal Society of Chemistry},
         journal = {MedChemComm},
            year = {2015},
           title = {Ionic liquid-mediated transcutaneous protein delivery with solid-in-oil nanodispersions},
             doi = {10.1039/c5md00378d},
          number = {12},
            note = {cited By 45},
          volume = {6},
        abstract = {As a potentially safe and non-invasive vaccination method, transcutaneous immunization represents an attractive alternative to conventional vaccine delivery by injection. However, the development of transcutaneous immunization has remained a challenge for a large number of hydrophilic macromolecules including protein and peptide antigens. We report a novel ionic liquid (IL)-mediated transcutaneous vaccine formulation consisting of a solid-in-oil (S/O) nanodispersion of antigen coated with pharmaceutically accepted surfactants dispersed in IL-containing oil. The introduction of the IL C12mimTf2N (1-dodecyl-3-methyl imidazolium bis(trifluoromethyl sulfonyl) amide) as a penetration enhancer in the formulation significantly enhanced the skin permeability of ovalbumin (OVA), a model antigen. It was also found that the IL-mediated S/O nanodispersion obtained high levels of OVA-specific serum IgG compared with both S/O nanodispersions without IL and PBS control. These findings clearly indicate that ILs-which are potentially attractive "green" and "designer" solvents-could serve as potential skin penetration enhancers in transcutaneous vaccination for hydrophilic macromolecules. {\^A}{\copyright} 2015 The Royal Society of Chemistry.},
        keywords = {1 dodecyl 3 methyl imidazolium bis(trifluoromethyl sulfonyl)amide; immunoglobulin G; ionic liquid; ovalbumin; penetration enhancing agent; surfactant; unclassified drug, animal experiment; animal tissue; Article; controlled study; dispersion; drug delivery system; drug formulation; female; immunization; immunoglobulin blood level; in vitro study; in vivo study; mouse; nonhuman; particle size; priority journal; skin permeability; solid in oil nanodispersion},
             url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84948741408&doi=10.1039\%2fc5md00378d&partnerID=40&md5=6582bb002708fb8fb345c2f98316ab7c},
            issn = {20402503},
          author = {Araki, S. and Wakabayashi, R. and Moniruzzaman, M. and Kamiya, N. and Goto, M.}
}