eprintid: 4664
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/00/46/64
datestamp: 2023-11-09 16:16:22
lastmod: 2023-11-09 16:16:22
status_changed: 2023-11-09 15:59:03
type: article
metadata_visibility: show
creators_name: Moniruzzaman, M.
creators_name: Nao, Y.
creators_name: Bhattacharjee, S.
creators_name: Ono, T.
title: Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media
ispublished: pub
keywords: Biological materials; Catalyst activity; Emulsification; Emulsions; Enzyme activity; Enzyme immobilization; Enzymes; Liquids; Polyesters; Polyethylene glycols; Polymers; Polyols; Scanning electron microscopy, Biological functions; Hexafluorophosphates; Identical conditions; Initial activity; Poly lactide; Polymer based composite; Storage stability; Water in oil emulsions, Ionic liquids
note: cited By 6; Conference of 3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014 ; Conference Date: 3 June 2014 Through 5 June 2014; Conference Code:114811
abstract: Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) C2mimPF6 (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions. © 2014 Trans Tech Publications, Switzerland.
date: 2014
publisher: Trans Tech Publications Ltd
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84914177548&doi=10.4028%2fwww.scientific.net%2fAMM.625.333&partnerID=40&md5=71cef0ccab8b919a5201ce522a346932
id_number: 10.4028/www.scientific.net/AMM.625.333
full_text_status: none
publication: Applied Mechanics and Materials
volume: 625
pagerange: 333-336
refereed: TRUE
isbn: 9783038351818
issn: 16609336
citation:   Moniruzzaman, M. and Nao, Y. and Bhattacharjee, S. and Ono, T.  (2014) Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media.  Applied Mechanics and Materials, 625.  pp. 333-336.  ISSN 16609336