TY  - JOUR
KW  - Biological materials; Catalyst activity; Emulsification; Emulsions; Enzyme activity; Enzyme immobilization; Enzymes; Liquids; Polyesters; Polyethylene glycols; Polymers; Polyols; Scanning electron microscopy
KW  -  Biological functions; Hexafluorophosphates; Identical conditions; Initial activity; Poly lactide; Polymer based composite; Storage stability; Water in oil emulsions
KW  -  Ionic liquids
ID  - scholars4664
N2  - Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) C2mimPF6 (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions. © 2014 Trans Tech Publications, Switzerland.
VL  - 625
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-84914177548&doi=10.4028%2fwww.scientific.net%2fAMM.625.333&partnerID=40&md5=71cef0ccab8b919a5201ce522a346932
A1  - Moniruzzaman, M.
A1  - Nao, Y.
A1  - Bhattacharjee, S.
A1  - Ono, T.
JF  - Applied Mechanics and Materials
Y1  - 2014///
SP  - 333
TI  - Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media
N1  - cited By 6; Conference of 3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014 ; Conference Date: 3 June 2014 Through 5 June 2014; Conference Code:114811
AV  - none
EP  - 336
SN  - 16609336
PB  - Trans Tech Publications Ltd
ER  -