%0 Journal Article
%@ 16609336
%A Moniruzzaman, M.
%A Nao, Y.
%A Bhattacharjee, S.
%A Ono, T.
%D 2014
%F scholars:4664
%I Trans Tech Publications Ltd
%J Applied Mechanics and Materials
%K Biological materials; Catalyst activity; Emulsification; Emulsions; Enzyme activity; Enzyme immobilization; Enzymes; Liquids; Polyesters; Polyethylene glycols; Polymers; Polyols; Scanning electron microscopy, Biological functions; Hexafluorophosphates; Identical conditions; Initial activity; Poly lactide; Polymer based composite; Storage stability; Water in oil emulsions, Ionic liquids
%P 333-336
%R 10.4028/www.scientific.net/AMM.625.333
%T Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media
%U https://khub.utp.edu.my/scholars/4664/
%V 625
%X Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) C2mimPF6 (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions. © 2014 Trans Tech Publications, Switzerland.
%Z cited By 6; Conference of 3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014 ; Conference Date: 3 June 2014 Through 5 June 2014; Conference Code:114811