relation: https://khub.utp.edu.my/scholars/4664/ title: Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media creator: Moniruzzaman, M. creator: Nao, Y. creator: Bhattacharjee, S. creator: Ono, T. description: Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) C2mimPF6 (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70% of its initial activity retained after 12 days of storage in IL at 40 °C, whereas it was about 20% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions. © 2014 Trans Tech Publications, Switzerland. publisher: Trans Tech Publications Ltd date: 2014 type: Article type: PeerReviewed identifier: Moniruzzaman, M. and Nao, Y. and Bhattacharjee, S. and Ono, T. (2014) Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media. Applied Mechanics and Materials, 625. pp. 333-336. ISSN 16609336 relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84914177548&doi=10.4028%2fwww.scientific.net%2fAMM.625.333&partnerID=40&md5=71cef0ccab8b919a5201ce522a346932 relation: 10.4028/www.scientific.net/AMM.625.333 identifier: 10.4028/www.scientific.net/AMM.625.333