@article{scholars4664,
            note = {cited By 6; Conference of 3rd International Conference on Process Engineering and Advanced Materials, ICPEAM 2014 ; Conference Date: 3 June 2014 Through 5 June 2014; Conference Code:114811},
          volume = {625},
             doi = {10.4028/www.scientific.net/AMM.625.333},
           title = {Laccase incorporated into PEG-PLA polymer as active and stable biocatalyst for ionic liquids media},
            year = {2014},
         journal = {Applied Mechanics and Materials},
       publisher = {Trans Tech Publications Ltd},
           pages = {333--336},
          author = {Moniruzzaman, M. and Nao, Y. and Bhattacharjee, S. and Ono, T.},
            issn = {16609336},
            isbn = {9783038351818},
             url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84914177548&doi=10.4028\%2fwww.scientific.net\%2fAMM.625.333&partnerID=40&md5=71cef0ccab8b919a5201ce522a346932},
        keywords = {Biological materials; Catalyst activity; Emulsification; Emulsions; Enzyme activity; Enzyme immobilization; Enzymes; Liquids; Polyesters; Polyethylene glycols; Polymers; Polyols; Scanning electron microscopy, Biological functions; Hexafluorophosphates; Identical conditions; Initial activity; Poly lactide; Polymer based composite; Storage stability; Water in oil emulsions, Ionic liquids},
        abstract = {Laccase Y20 (EC.1.10.3.2) was coated with poly (ethylene glycol)-block-polylactide (PEG-PLA, MW = 27680) via water-in-oil emulsion, and the activity and stability of the resulting PEG-PLA-laccase complex have been compared to those for the native laccase and lyophilized native laccase in an ionic liquid (IL) C2mimPF6 (1-ethyl-3-methylimidazolium hexafluorophosphate. The formation of spherical PEG-PLA-laccase complex of 330- 480 nm was demonstrated by scanning electron microscopy. This polymer-laccase complex retained most of its enzymatic catalytic activity and exhibited excellent storage stability in IL, with over 70\% of its initial activity retained after 12 days of storage in IL at 40 {\^A}oC, whereas it was about 20\% for native laccase under the identical conditions. This strategy could be employed to fabricate polymer based composites materials with novel biological functions. {\^A}{\copyright} 2014 Trans Tech Publications, Switzerland.}
}