@article{scholars4057,
       publisher = {Elsevier Ltd},
         journal = {Bioresource Technology},
           pages = {132--137},
            year = {2013},
           title = {Separation and characterization of cellulose fibers from cypress wood treated with ionic liquid prior to laccase treatment},
          volume = {127},
            note = {cited By 152},
             doi = {10.1016/j.biortech.2012.09.113},
        keywords = {Acetone; Cellulose; Delignification; Ionic liquids; Lignin; Separation; Textile fibers, Cellulose content; Cellulose crystallinity; Cellulose fiber; Chemical compositions; Chemical method; Enzymatic delignification; FTIR; Laccase treatment; Laccases; Lignin contents; Mixture priors; Pre-Treatment; Structural alterations; Wood biomass; Wood materials; Woody biomass; XRD, Wood, 1 ethyl 3 methylimidazolium acetate; 1 hydroxybenzotriazole; acetic acid derivative; acetone; benzotriazole derivative; cellulose; ionic liquid; laccase; lignin; unclassified drug; water, biomass; cellulose; chemical composition; crystallinity; enzyme; ion; lignin; separation; wood, analytic method; article; biomass; chemical composition; controlled study; crystal structure; crystallization; fiber; infrared spectroscopy; nonhuman; priority journal; scanning electron microscopy; separation technique; surface property; thermogravimetry; thermostability; Trametes; wood; X ray diffraction, Acetone; Biomass; Cellulose Fibers; Delignification; Ions; Laccase; Lignins; Separation; Textile Fibers; Wood, Cupressus; Trametes},
             url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84868244778&doi=10.1016\%2fj.biortech.2012.09.113&partnerID=40&md5=1bb33f4ae8711db4500ae6b327f1f9de},
        abstract = {Separation of cellulose fibers (CFs) from woody biomass with minimal structural alteration using a "green" and efficient method was achieved by treatment with the ionic liquid (IL), emimOAc (1-ethyl-3-methylimidazolium acetate) at 80. {\^A}oC for 1. h. The IL was recovered by rinsing with water-acetone mixture prior to treatment of the wood with Trametes sp. laccase in the presence of 1-hydroxybenzotriazole as a mediator. IL pretreatment did not significantly change the chemical composition of the wood, but did alter its structure and rendered its surface more accessible to the enzyme. Treated and untreated samples were characterized by SEM, FTIR, XRD, TGA, and chemical methods. The cellulose content of the produced fibers was approximately 73.1\% and the lignin content was 9.8\%, much lower than the lignin content of 29.3\% of the untreated wood. The cellulose fibers exhibited higher cellulose crystallinity and better thermal stability compared to untreated wood materials. {\^A}{\copyright} 2012 Elsevier Ltd.},
            issn = {09608524},
          author = {Moniruzzaman, M. and Ono, T.}
}