TY  - JOUR
AV  - none
N2  - The effect of phase forming components, pH, neutral salt (NaCl) addition on partitioning of bovine serum albumin (BSA) in PEG2000-sodium citrate based aqueous two-phase system was investigated at 25, 35, and 45C. The systems were prepared by varying the phase forming components concentration at a pH of 5.0, 6.0, 7.0, 8.0, and 9.0 with different NaCl salt concentrations of 0.05M, 0.1M, 1.5M, 0.2M, 0.3M, and 0.4M. The affinity of the BSA for the lower phase increases with increase in pH, due to increase in ratio of trivalent to divalent citrate ions in the two-phase system. It was confirmed that the partition of BSA depends on the relative hydrophobicity of the compounds as well as their charge. Optimum level of pH and NaCl concentration for the partitioning of bovine serum albumin was determined.
VL  - 42
JF  - Separation Science and Technology
KW  - Concentration (process); Hydrophobicity; Optimization; pH effects; Sodium chloride; Solutions; Water
KW  -  Bovine serum albumin; Partitioning; Polyethylene glycol; Sodium citrate
KW  -  Two phase flow
KW  -  Concentration (process); Hydrophobicity; Optimization; pH effects; Sodium chloride; Solutions; Two phase flow; Water
KW  -  concentration; hydrophobic property; pH
EP  - 2065
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547404303&doi=10.1080%2f15363830701313404&partnerID=40&md5=6d48ba92c9c90f3e11701757c0989413
N1  - cited By 13
SP  - 2049
IS  - 9
TI  - Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system
SN  - 01496395
ID  - scholars281
A1  - Perumalsamy, M.
A1  - Murugesan, T.
Y1  - 2007///
ER  -