eprintid: 255
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/00/02/55
datestamp: 2023-11-09 15:15:53
lastmod: 2023-11-09 15:15:53
status_changed: 2023-11-09 15:13:39
type: article
metadata_visibility: show
creators_name: Chiang, L.
creators_name: Abdullah, M.A.
title: Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
ispublished: pub
keywords: Adsorbents; Adsorption; Growth kinetics; Solvents; Suspensions (fluids), Amberlite polymeric adsorbent; Anthraquinones; Morinda elliptica, Cell culture, Morinda; Morinda elliptica
note: cited By 31
abstract: Continuous removal of anthraquinones (AQ) by Amberlite polymeric adsorbents (XAD-4, XAD-7 and XAD-16) through in situ adsorption in Morinda elliptica cell suspension cultures is studied for product recovery and improvement of the overall titre. Ethanol was the best eluting solvent for effective recovery of AQ from all adsorbents. Pre-treatment of XAD-4 with sodium acetate not only enhanced intracellular AQ, but also AQ release and subsequent recovery from the adsorbent. The addition of sodium acetate pretreated XAD-4 on day 18 for 6-day contact period, achieved comparable cell growth to control (41 g/L), but with 1.3-fold higher intracellular AQ (124 mg/g DW) and two-fold increase in extracellular AQ (14.3 mg/L). High amount of adsorbent and longer contact period for the cultures entering stationary growth phase, stimulated AQ release and recovery but at the expense of cell growth. With 5-8.3 g XAD-4 adsorbent per litre M. elliptica culture in production (P) medium, between 60 and 90 AQ was recovered from extracellular AQ after 24-26 days of culture period. © 2007 Elsevier Ltd. All rights reserved.
date: 2007
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-34047104562&doi=10.1016%2fj.procbio.2007.01.005&partnerID=40&md5=971e3a7393e308d82f58b10ac31c7584
id_number: 10.1016/j.procbio.2007.01.005
full_text_status: none
publication: Process Biochemistry
volume: 42
number: 5
pagerange: 757-763
refereed: TRUE
issn: 13595113
citation:   Chiang, L. and Abdullah, M.A.  (2007) Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy.  Process Biochemistry, 42 (5).  pp. 757-763.  ISSN 13595113