relation: https://khub.utp.edu.my/scholars/20258/
title: Cyclic and differential pulse voltammetric measurements on fibrils formation of alpha synuclein in Parkinson's disease by a gold interdigitated tetraelectrodes
creator: Adam, H.
creator: Gopinath, S.C.B.
creator: Krishnan, H.
creator: Adam, T.
creator: Mohammed, M.
creator: Perumal, V.
creator: Fakhri, M.A.
creator: Salim, E.T.
creator: Raman, P.
creator: Subramaniam, S.
creator: Chen, Y.
creator: Sasidharan, S.
description: Parkinson's disease is a neurodegenerative disorder characterized by the aggregation and deposition of alpha-synuclein protein, which are pathological hallmarks. To understand the fibril formation of alpha-synuclein in Parkinson's disease, this study uses cyclic and differential pulse voltammetric measurements. These measurements analyze the electrochemical properties and behavior of alpha-synuclein during its fibril formation process. By applying a potential sweep or pulse to the alpha-synuclein sample, it is possible to gain insights into its redox activity and structural changes during fibril formation. This could lead to the development of therapeutic strategies to prevent or disrupt this pathological event in Parkinson's disease. To detect Parkinson's disease, a 15 nm sized gold conjugated antibody was used as the probe and seeded on gold interdigitated tetraelectrodes (AuIDTE). Alpha synuclein variations (fibriled and non-fibriled) were detected using phosphate-buffer saline and glycine buffer based on cyclic voltammetry and differential pulse voltammetry techniques. Discriminated by Tau protein measurement that was employed as a control. The linear regression for detecting alpha synuclein aggregation using differential pulse voltammetry was R2 = 0.9987 y = 9E-06x - 4E-07, with a limit of detection of 10 aM, on a linear range of 1 aM-1 pM. Cyclic voltammetry determined the limit of detection of aggregated alpha synuclein to be 100 aM, with a linear relationship of R2 = 0.9939 y = 7E-06x - 2E-06. The sensor has excellent analytical performance in terms of detection limit, sensitivity, selectivity, repeatability, and stability. © 2023 Elsevier Ltd
publisher: Elsevier Ltd
date: 2024
type: Article
type: PeerReviewed
identifier:   Adam, H. and Gopinath, S.C.B. and Krishnan, H. and Adam, T. and Mohammed, M. and Perumal, V. and Fakhri, M.A. and Salim, E.T. and Raman, P. and Subramaniam, S. and Chen, Y. and Sasidharan, S.  (2024) Cyclic and differential pulse voltammetric measurements on fibrils formation of alpha synuclein in Parkinson's disease by a gold interdigitated tetraelectrodes.  Process Biochemistry, 136.  pp. 212-220.  ISSN 13595113     
relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85179754994&doi=10.1016%2fj.procbio.2023.11.019&partnerID=40&md5=dbe540ac42dba41c69ed61679ebcd19d
relation: 10.1016/j.procbio.2023.11.019
identifier: 10.1016/j.procbio.2023.11.019