%A A.A.M. Elgharbawy %A S.S. Syed Putra %A H.W. Khan %A N.A.N. Azmi %A M.S.A. Sani %A N. Ab llah %A A. Hayyan %A J. Jewaratnam %A W.J. Basirun %D 2023 %N 2 %O cited By 6 %X This research aims to provide insights into the biological efficacy of a newly formed hydrophobic deep eutectic solvent (DES). A DES based on menthol was successfully synthesized with fatty acids. The DESs� properties as enzyme activators were examined against a neat counterpart. The menthol:decanoic acid (1:1) combination showed improved thermal stability, strong catalytic activity, and reusability for up to four subsequent cycles under ideal conditions (pH 7.0, 40 °C for 2 h). The hydrophobic DES replaced hexane in ester synthesis, where RNL@DES5 showed better fatty acid conversion compared to neat RNL. This study demonstrated promising applications of hydrophobic DESs in non-aqueous organic reactions. © 2023 by the authors. %K Catalyst activity; Eutectics; Fatty acids; Hydrogen; Hydrogen bonds; Hydrophobicity; Monoterpenes; Reusability; Solvents, Decanoic acid; Deep eutectic solvents; Enzyme activation; Enzymes kinetics; Hydrophobic agents; Hydrophobics; Natural deep eutectic solvent; Property; Solvent based; Synthesised, Enzyme kinetics %L scholars18834 %R 10.3390/pr11020547 %V 11 %J Processes %T Menthol and Fatty Acid-Based Hydrophobic Deep Eutectic Solvents as Media for Enzyme Activation