TY - JOUR EP - 451 SN - 09601481 PB - Elsevier Ltd N1 - cited By 29 SP - 443 TI - Dual nutrient heterogeneity modes in a continuous flow photobioreactor for optimum nitrogen assimilation to produce microalgal biodiesel AV - none UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120639650&doi=10.1016%2fj.renene.2021.11.117&partnerID=40&md5=c9daf83d536280c8b27f8639e3e9f6c7 JF - Renewable Energy A1 - Leong, W.H. A1 - Kiatkittipong, W. A1 - Lam, M.K. A1 - Khoo, K.S. A1 - Show, P.L. A1 - Mohamad, M. A1 - Chong, S. A1 - Abdurrahman, M. A1 - Lim, J.W. VL - 184 Y1 - 2022/// N2 - The impact of different nitrogen sources on the microalga, Chlorella vulgaris, was studied in a newly developed microalgal-bacterial photobioreactor via a dual nutrient heterogeneity mode. The mechanisms of nitrogen transformation and valorisation were unveiled, and subsequently, optimized via dual nutrient heterogeneity feeding modes comprising of various NH4+-N and NO3â??-N concentrations. The nitrogen-assimilation mechanism from the microalgal-bacterial consortium was found to reduce microalgal growth inhibition, stemming at high NH4+-N concentrations. Accordingly, the total nitrogen removal efficiency was enhanced from 40.91 to 96.38 accompanied with maximum microalgal biomass production of up to 792 mg/L at a balanced or higher NH4+-N loading from mixed nitrogen environment. The harvested microalgal biomass contained high lipid accumulation of approximately 30 when fed with optimum NH4+-N and NO3â??-N loadings at 60 and 58 mg/d, respectively. At this mixed nitrogen species loadings, high unsaturated fatty acid methyl esters (FAME) were attained. Indeed, the major FAME species (97â??100) fell within the C16-18 range, signifying biodiesel characteristics conformity to the requirements for quality biodiesel application. Therefore, the dual heterogeneity modes of balanced NH4+-N and NO3â??-N loadings into photobioreactor could offer effective microalgal nitrogen assimilation for sustainable wastewater treatment and microalgae-based biodiesel production simultaneously. © 2021 Elsevier Ltd KW - Biodiesel; Microorganisms; Nitrogen removal; Nutrients; Photobioreactors; Unsaturated fatty acids; Wastewater treatment KW - Activated sludge; Continuous-flow; Fatty acids methyl esters; Microalga; Microalgal biodiesels; Microalgal biomass; NH 4 + N; Nitrogen assimilation; Nitrogen sources; Photobiore-actor KW - Microalgae KW - biofuel; biomass; fatty acid; heterogeneity; lipid; nutrient; valorization; wastewater; wastewater treatment KW - Bacteria (microorganisms); Chlorella vulgaris ID - scholars17818 ER -