TY  - JOUR
N1  - cited By 3
N2  - Biobased ternary nanocomposites can stabilize enzymes for greater stability, catalytic activity and easy recovery. This study aimed to optimize biogenic silica/magnetite/graphene oxide nanocomposite supported Candida rugosa lipase (CRL/SiO2/Fe3O4/GO) for ethyl valerate (EV) synthesis and characterize the biocatalystsâ?? physicochemical properties and operational stability. CRL conjugated-oil palm leaves-derived biogenic SiO2/Fe3O4/GO nanocomposite showed a maximum immobilized protein of 44.13 ± 2.1 mg/g with a specific activity (534.87 ± 9.5 U/mg), than free CRL (â?¥700 U/mg). GL-A-SiO2/Fe3O4/GO exhibited the highest surface area (260.87 m2/g) alongside superior thermal stability in TGA/DTG. XRD revealed an amorphous SiO2 (crystallinity = 26.7), while Fe3O4 existed as cubic spinel crystal (crystallinity = 90.2). Taguchi Design-optimization found that CRL/SiO2/Fe3O4/GO best catalyzed the EV synthesis (90.4 in 3 h) at 40 â?? using 3 mg/mL of biocatalyst, valeric acid/ethanol molar ratio of 1:2, in 10 (m/v) molecular sieves with stirring in heptane at 200 rpm. EV production was confirmed by FTIR- (C=O: 1738 cmâ??1 and Câ??Oâ??C: 1174 cmâ??1) and GCâ??MS (M+ m/z = 130, C7H14O2). CRL/SiO2/Fe3O4/GO's reusability for 11 successive esterification cycles demonstrated the SiO2/Fe3O4/GO's exceptional hyperactivation and stabilization properties on immobilized CRL. These findings conveyed the SiO2/Fe3O4/GO's efficacy to alter CRL's physicochemical properties and operational stability for catalyzing higher yields EV. © 2021 The Society of Powder Technology Japan
IS  - 1
ID  - scholars17817
TI  - Physicochemical properties and operational stability of Taguchi design-optimized Candida rugosa lipase supported on biogenic silica/magnetite/graphene oxide for ethyl valerate synthesis
KW  - Candida; Catalyst activity; Crystallinity; Esterification; Esters; Fatty acids; Lipases; Molar ratio; Molecular sieves; Nanocomposites; Palm oil; Physicochemical properties; Reusability; Silica; Silicon; Stability; Yeast
KW  -  Biocatalyst characterization; Biogenic silica; Candida rugosa lipase; Esterification synthesis; Ethyl valerate; Nanocomposite support; Operational stability; Physicochemical property; Taguchi design; Taguchi optimizations
KW  -  Magnetite
AV  - none
JF  - Advanced Powder Technology
A1  - Jacob, A.G.
A1  - Wahab, R.A.
A1  - Chandren, S.
A1  - Jumbri, K.
A1  - Wan Mahmood, W.M.A.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120705367&doi=10.1016%2fj.apt.2021.11.026&partnerID=40&md5=b4e84497f36c33343a7ffff74b381864
VL  - 33
Y1  - 2022///
PB  - Elsevier B.V.
SN  - 09218831
ER  -