%V 69
%J Biotechnology and Applied Biochemistry
%R 10.1002/bab.2116
%T Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor
%O cited By 1
%D 2022
%I John Wiley and Sons Inc
%X Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface. (3-Aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve y = 0.1311x + 0.5386; R² = 0.9707 with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and nonimmune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer. © 2021 International Union of Biochemistry and Molecular Biology, Inc.
%L scholars17164
%A Y. Wang
%A X. Sun
%A S.C.B. Gopinath
%A M.S.M. Saheed
%A X. Wang
%P 376-382
%N 1
%K Antibodies; Chemical detection; Diseases; Glycoproteins; Integrodifferential equations, Endocrine cancer; Endocrine glands; Functionalized; Immunoassay; Inter-digitated electrodes; Interdigitated electrodes; Silane coupling; Thyroglobulin; Thyroid cancers; Thyroid tumors, Electrochemical sensors, 3-Aminopropyl) triethoxysilane; alcohol; aluminum; antibody; chemical compound; distilled water; ethanolamine; phosphate buffered saline; silane antibody; silicon dioxide; thyroglobulin; thyroglobulin antibody; thyroid peroxidase; unclassified drug; silane derivative; thyroglobulin, antigen antibody complex; antigen antibody reaction; Article; female; human; immobilization; limit of detection; male; oxidation; pH; room temperature; thermal oxidation; thyroglobulin blood level; thyroid cancer; thyroid gland; thyroid tumor; ultraviolet radiation; electrode, Electrodes; Humans; Silanes; Thyroglobulin; Thyroid Neoplasms