@article{scholars16351, title = {Ultrasensitive aptasensor using electrospun MXene/polyvinylidene fluoride nanofiber composite for Ochratoxin A detection}, journal = {Food Chemistry}, publisher = {Elsevier Ltd}, volume = {390}, note = {cited By 23}, doi = {10.1016/j.foodchem.2022.133105}, year = {2022}, issn = {03088146}, author = {Al-Dhahebi, A. M. and Jose, R. and Mustapha, M. and Saheed, M. S. M.}, keywords = {Charge transfer; Electrochemical electrodes; Fruit juices; Nanofibers, Aptasensors; Biosensor design; Electroactive material; Electrospuns; Food samples; Mycotoxin contamination; Nanofiber composites; Ochratoxin A; Piezoelectric sensors; Ultrasensitive, Fluorine compounds, aldehyde; aptamer; nanofiber; ochratoxin; polyvinylidene fluoride; sodium chloride; titanium carbide; aptamer; fluorocarbon; ochratoxin; polyvinyl derivative; polyvinylidene fluoride, Article; electrospinning; food industry; grape juice; limit of detection; limit of quantitation; electrochemical analysis; genetic procedures, Aptamers, Nucleotide; Biosensing Techniques; Electrochemical Techniques; Fluorocarbon Polymers; Limit of Detection; Nanofibers; Ochratoxins; Polyvinyls}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130371663&doi=10.1016\%2fj.foodchem.2022.133105&partnerID=40&md5=86fc5c616235a8266264b126078d8dda}, abstract = {Ochratoxin A (OTA) has harmful effects to human and animal health; therefore, sensitive and selective detection of OTA is highly demanded. Herein, an ultrasensitive electrochemical aptasensor electrode comprising electrospun MXene/polyvinylidene fluoride (Ti3C2Tx/PVDF) nanofiber composite is presented. Addition of Ti3C2Tx up to 13 effectively increased the fiber diameter and lowered the {\^I}2-phase of PVDF nanofibers, consequently lowering the charge transfer resistance. The nanofiber composite is then coated on the screen-printed carbon electrode to chemically functionalized with saline and aldehyde groups for efficient aptamer loading. The optimized aptasensor demonstrated sensitive detection of OTA over the dynamic concentration range from 1 fg mL{\^a}??1 to 1 ng mL{\^a}??1 with a limit of detection of 2.15 fg mL{\^a}??1 and quantification limit of 6.52 fg mL{\^a}??1, with high selectivity. The aptasensor could detect the OTA at femtogram per milliliter concentration in grape juice samples, demonstrating its enormous potential for OTA detection in food industry. {\^A}{\copyright} 2022 Elsevier Ltd} }