relation: https://khub.utp.edu.my/scholars/16261/
title: Microbial degradation and transformation of benzoapyrene by using a white-rot fungus Pleurotus eryngii F032
creator: Hadibarata, T.
creator: Kristanti, R.A.
creator: Bilal, M.
creator: Al-Mohaimeed, A.M.
creator: Chen, T.-W.
creator: Lam, M.K.
description: Polycyclic aromatic hydrocarbons (PAHs) are environmentally recalcitrant contaminants formed from naturally or incomplete combustion of organic materials and some of them are difficult to degrade due to their hydrophobicity and persistency. Benzo apyrene (BaP), is one of PAHs that having five fused benzene and reported as mutagenic, carcinogenic and teratogenic compounds. Biodegradation is one of promising techniques due to its relatively low economic cost and microorganism is a natural capacity to consume hydrocarbons. In this investigation, Pleurotus eryngii F032 was grown in 20 mL of modified mineral salt broth (MSB) supplemented with BaP under static and agitated culture. Within 20 days, static culture removed 59% of BaP, whereas agitated culture removed the highest amount (73%). To expedite BaP elimination, the mechanism and behavior of BaP biosorption and biotransformation by Pleurotus eryngii F032 were additionally examined by gas chromatography-mass spectrometer (GC-MS). The optimal conditions for P. eryngii F032 to eliminate BaP were 25 °C, a C/N ratio of 8, pH 3 and 0.2% inoculum concentration. At an initial BaP content of 10 mg/L, more than 50% was effectively eliminated within 20 days under these conditions. Salinity, glucose, and rhamnolipids were the most important factors impacting BaP biodegradation. GC-MS found degradation products such as BaP-3,6-quinone, indicating plausible metabolic routes. Finally, it may be assumed that the primary mechanism by which white-rot fungi eliminate BaP is by the utilization of biotransformation enzymes such as laccase to mineralize the PAHs. Hence, Pleurotus eryngii F032 could be an ideal candidate to treat PAHs contaminated soils. © 2022 Elsevier Ltd
publisher: Elsevier Ltd
date: 2022
type: Article
type: PeerReviewed
identifier:   Hadibarata, T. and Kristanti, R.A. and Bilal, M. and Al-Mohaimeed, A.M. and Chen, T.-W. and Lam, M.K.  (2022) Microbial degradation and transformation of benzoapyrene by using a white-rot fungus Pleurotus eryngii F032.  Chemosphere, 307.   ISSN 00456535     
relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135926849&doi=10.1016%2fj.chemosphere.2022.136014&partnerID=40&md5=cc098ed6c87b521e5ee8bc172a556b05
relation: 10.1016/j.chemosphere.2022.136014
identifier: 10.1016/j.chemosphere.2022.136014