eprintid: 16241 rev_number: 2 eprint_status: archive userid: 1 dir: disk0/00/01/62/41 datestamp: 2023-12-19 03:22:47 lastmod: 2023-12-19 03:22:47 status_changed: 2023-12-19 03:05:54 type: article metadata_visibility: show creators_name: Chong, S.L. creators_name: Tan, I.S. creators_name: Foo, H.C.Y. creators_name: Chan, Y.S. creators_name: Lam, M.K. creators_name: Lee, K.T. title: Ultrasonic�assisted molten salt hydrates pretreated Eucheuma cottonii residues as a greener precursor for third-generation L-lactic acid production ispublished: pub keywords: Chlorine compounds; Hydrates; Hydration; Hydrolysis; Lactic acid, Dilute-acid hydrolysis; Eucheuma cottonii; Eucheuma cottonii residue; Green salt treatment; L-lactic acids; Molten salt; Salt hydrates; Salts treatment; Separate hydrolysis and fermentation; Yeast extracts, Glucose, glucose; lactic acid; nicotinamide; pantothenic acid; yeast extract, bacterium; fermentation; glucose; hydrolysis; substrate; vitamin, acid hydrolysis; acidity; Article; biofuel production; biomass energy; carbon nuclear magnetic resonance; cell density; chemical structure; controlled study; crystal structure; dissolution; fermentation; high temperature; hybrid; hydrogen bond; inoculation; Kappaphycus alvarezii; liquefaction; low temperature; macroalga; microbial biomass; micromorphology; molecular weight; nonhuman; precursor; product quality; proton nuclear magnetic resonance; reaction temperature; reaction time; structure analysis; ultrasound; viscosity note: cited By 0 abstract: This study aims to establish an efficient pretreatment method that facilitates the conversion of sugars from macroalgae wastes, Eucheuma cottonii residues (ECRs) during hydrolysis and subsequently enhances L-lactic acid (L-LA) production. Hence, ultrasonic-assisted molten salt hydrates (UMSHs) pretreatment was proposed to enhance the accessibility of ECRs to hydrolyze into glucose through dilute acid hydrolysis (DAH). The obtained hydrolysates were employed as the substrate in producing L-LA by separate hydrolysis and fermentation (SHF). The maximum glucose yield (97.75 ) was achieved using UMSHs pretreated ECRs with 40 wt ZnCl2 at 80 °C for 2 h and followed with DAH. The optimum glucose to L-LA yield obtained for SHF was 90.08 using 5 (w/w) inoculum cell densities of B. coagulans ATCC 7050 with yeast extract (YE). A comparable performance (89.65 ) was obtained using a nutrient combination (lipid-extracted Chlorella vulgaris residues (CVRs), vitamin B3, and vitamin B5) as a partial alternative for YE. © 2022 Elsevier Ltd date: 2022 publisher: Elsevier Ltd official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140083701&doi=10.1016%2fj.biortech.2022.128136&partnerID=40&md5=090c7386744deb504f9d2c0248ea5681 id_number: 10.1016/j.biortech.2022.128136 full_text_status: none publication: Bioresource Technology volume: 364 refereed: TRUE issn: 09608524 citation: Chong, S.L. and Tan, I.S. and Foo, H.C.Y. and Chan, Y.S. and Lam, M.K. and Lee, K.T. (2022) Ultrasonic�assisted molten salt hydrates pretreated Eucheuma cottonii residues as a greener precursor for third-generation L-lactic acid production. Bioresource Technology, 364. ISSN 09608524