relation: https://khub.utp.edu.my/scholars/15958/ title: Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from �mam nem�, a fermented fish sauce creator: Thuy, D.T.B. creator: Nguyen, A. creator: Khoo, K.S. creator: Chew, K.W. creator: Cnockaert, M. creator: Vandamme, P. creator: Ho, Y.-C. creator: Huy, N.D. creator: Cocoletzi, H.H. creator: Show, P.L. description: This study was aimed to identify and optimize the culture conditions for gamma-aminobutyric acid (GABA) production by a lactic acid bacterium strain isolated from mam nem, a fermented fish sauce. Among the six isolates obtained from mam nem, the MN12 had the most potent GABA-producing capability. The strain was then identified to be Pedioccocus pentosaceus by employing MALDI-TOF-MS and phenylalanyl-tRNA synthase sequencing methods. The initial cell density of 5.106 CFU/mL, monosodium glutamate concentration of 60 mM, initial pH of 7, temperature of 45°C and cultivation time of 72 h were found to be the optimal culture conditions for highest production of GABA, reaching 27.9 ± 0.42 mM, by this strain. The cultivation conditions for GABA production by P. pentosaceus MN12 have been successfully optimized, providing a foundation for the development of fermented foods enriched with GABA. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. publisher: Bellwether Publishing, Ltd. date: 2021 type: Article type: PeerReviewed identifier: Thuy, D.T.B. and Nguyen, A. and Khoo, K.S. and Chew, K.W. and Cnockaert, M. and Vandamme, P. and Ho, Y.-C. and Huy, N.D. and Cocoletzi, H.H. and Show, P.L. (2021) Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from �mam nem�, a fermented fish sauce. Bioengineered, 12 (1). pp. 54-62. ISSN 21655979 relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097933074&doi=10.1080%2f21655979.2020.1857626&partnerID=40&md5=726ebca0f6f70d042b30f98f1e98d48c relation: 10.1080/21655979.2020.1857626 identifier: 10.1080/21655979.2020.1857626