TY - JOUR PB - Taylor and Francis Ltd. SN - 17597269 EP - 828 AV - none N1 - cited By 2 TI - High yield of second-generation ethanol in an ionic Liquid-Cellulase integrated system for single-step processing of empty fruit bunch SP - 817 Y1 - 2021/// A1 - Elgharbawy, A.A.M. A1 - Alam, M.D.Z. A1 - Moniruzzaman, M. A1 - Ahmad Kabbashi, N. A1 - Jamal, P. JF - Biofuels UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109075377&doi=10.1080%2f17597269.2018.1542569&partnerID=40&md5=758916660d6ec5008977ad558f818415 VL - 12 N2 - Ionic liquids (ILs) are regarded as green solvents and have demonstrated ability in the solvation of lignocellulosic material, thus promoting its enzymatic hydrolysis by cellulases. In this study, cellulase (PKC-Cel) obtained from Trichoderma reesei (RUTC30) through fermentation displayed good stability in choline acetate ChoOAc, a biodegradable IL. Empty fruit bunches (EFB) were subjected to single-step hydrolysis in an IL-enzyme system. Response surface methodology was employed to maximize ethanol production. Three parameters were optimized, namely sugar loading, inoculum concentration, and agitation. The distilled ethanol purity was 98.6% (v/v). The ethanol yield was 87.94% of the theoretical yield, which was 0.275 g ethanol gâ??1 EFB. In comparison, the non-IL system produced 0.127 g gâ??1 EFB. The yield coefficients (Formula presented.), (Formula presented.), and (Formula presented.) were 0.552, 0.148, and 3.69 g gâ??1. The specific growth rate (Formula presented.) was determined to be 0.264 hâ??1 and the (Formula presented.) was 0.777 hâ??1. © 2019 Informa UK Limited, trading as Taylor & Francis Group. IS - 7 ID - scholars15727 KW - Cellulose derivatives; Ethanol; Fruits; Ionic liquids KW - Empty fruit bunches; Ethanol production; Inoculum concentrations; Integrated systems; Lignocellulosic material; Response surface methodology; Specific growth rate; Trichoderma reesei KW - Enzymatic hydrolysis ER -