relation: https://khub.utp.edu.my/scholars/15727/ title: High yield of second-generation ethanol in an ionic Liquid-Cellulase integrated system for single-step processing of empty fruit bunch creator: Elgharbawy, A.A.M. creator: Alam, M.D.Z. creator: Moniruzzaman, M. creator: Ahmad Kabbashi, N. creator: Jamal, P. description: Ionic liquids (ILs) are regarded as green solvents and have demonstrated ability in the solvation of lignocellulosic material, thus promoting its enzymatic hydrolysis by cellulases. In this study, cellulase (PKC-Cel) obtained from Trichoderma reesei (RUTC30) through fermentation displayed good stability in choline acetate ChoOAc, a biodegradable IL. Empty fruit bunches (EFB) were subjected to single-step hydrolysis in an IL-enzyme system. Response surface methodology was employed to maximize ethanol production. Three parameters were optimized, namely sugar loading, inoculum concentration, and agitation. The distilled ethanol purity was 98.6% (v/v). The ethanol yield was 87.94% of the theoretical yield, which was 0.275 g ethanol g�1 EFB. In comparison, the non-IL system produced 0.127 g g�1 EFB. The yield coefficients (Formula presented.), (Formula presented.), and (Formula presented.) were 0.552, 0.148, and 3.69 g g�1. The specific growth rate (Formula presented.) was determined to be 0.264 h�1 and the (Formula presented.) was 0.777 h�1. © 2019 Informa UK Limited, trading as Taylor & Francis Group. publisher: Taylor and Francis Ltd. date: 2021 type: Article type: PeerReviewed identifier: Elgharbawy, A.A.M. and Alam, M.D.Z. and Moniruzzaman, M. and Ahmad Kabbashi, N. and Jamal, P. (2021) High yield of second-generation ethanol in an ionic Liquid-Cellulase integrated system for single-step processing of empty fruit bunch. Biofuels, 12 (7). pp. 817-828. ISSN 17597269 relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109075377&doi=10.1080%2f17597269.2018.1542569&partnerID=40&md5=758916660d6ec5008977ad558f818415 relation: 10.1080/17597269.2018.1542569 identifier: 10.1080/17597269.2018.1542569