@inproceedings{scholars15024,
           title = {Cultivation of Chlorella vulgaris in Sequential Flow Photobioreactor System: Influence of Recycled Culture Medium on Growth, Lipid and Protein Content},
             doi = {10.1088/1755-1315/721/1/012013},
          volume = {721},
            note = {cited By 8; Conference of 2021 5th International Conference on Energy and Environmental Science, ICEES 2021 ; Conference Date: 8 January 2021 Through 10 January 2021; Conference Code:168314},
          number = {1},
         journal = {IOP Conference Series: Earth and Environmental Science},
       publisher = {IOP Publishing Ltd},
            year = {2021},
            issn = {17551307},
          author = {Dasan, Y. K. and Lam, M. K. and Yusup, S. and Lim, J. W. and Lee, K. T. and Show, P. L. and Tan, I. S. and Yew Foo, H. C.},
        abstract = {In the present work, the reusability of culture medium to grow Chlorella vulgaris was examined in an effort to reduce the freshwater footprint of microalgae cultivation. The microalagae was cultivated in a sequential-flow system equipped with five photobioreactors connected in series. Furthermore, the effect of recycled culture medium without additional nutrients on the growth, lipid and protein content of microalgae was evaluated under optimized cultivation conditions. Experimental results showed that biomass concentration and growth rate reduced when the recycled culture medium was used. The maximum biomass concentration was achieved in the fresh medium with a total biomass yield of 1.42 g/L. Meanwhile, lipid content was found to increase when the microalgae was cultivated in the recycled medium, in which the highest lipid content (58.01 wt.) was attained from photobioreactor 5 (cycle 2). On the contrary, the protein content (11.98 wt.) of the microalgae in the recycled medium was considerably lower than the fresh medium (49.20 wt.). These results suggested that the reuse of culture medium to grow microalgae is possible, however, it has a significant impact on the biochemical compositions of Chlorella vulgaris. Therefore, it is important to determine the desired product prior to reusing the medium for subsequent cultivation process. {\^A}{\copyright} Published under licence by IOP Publishing Ltd.},
        keywords = {Algae; Biomass; Ecology; Microorganisms; Photobioreactors; Proteins; Recycling; Reusability, Biochemical composition; Biomass concentrations; Chlorella vulgaris; Cultivation conditions; Cultivation process; Microalgae cultivation; Photobiore-actor; Protein contents, Cultivation},
             url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104184830&doi=10.1088\%2f1755-1315\%2f721\%2f1\%2f012013&partnerID=40&md5=9a506cfee719fe43050b6cfe14269812}
}