TY  - JOUR
N1  - cited By 7
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101363477&doi=10.1016%2fj.saa.2021.119543&partnerID=40&md5=8ba4204cf3a6987b95767a6d3e7db4bb
A1  - Yusof, R.
A1  - Jumbri, K.
A1  - Ahmad, H.
A1  - Abdulmalek, E.
A1  - Abdul Rahman, M.B.
ID  - scholars14935
N2  - The binding characteristics of DNA in deep eutectic solvents (DESs), particularly the binding energy and interaction mechanism, are not widely known. In this study, the binding of tetrabutylammonium bromide (TBABr) based DES of different hydrogen bond donors (HBD), including ethylene glycol (EG), glycerol (Gly), 1,3-propanediol (1,3-PD) and 1,5-pentanediol (1,5-PD), to calf thymus DNA was investigated using fluorescence spectroscopy. It was found that the shorter the alkyl chain length (2 carbons) and higher EG ratios of TBABr:EG (1:5) increased the binding constant (Kb) between DES and DNA up to 5.75 Ă? 105 kJ molâ??1 and decreased the binding of Gibbs energy (Î?Go) to 32.86 kJ molâ??1. Through displacement studies, all synthesised DESs have been shown to displace DAPI (4â?˛,6-diamidino-2-phenylindole) and were able to bind on the minor groove of Adenine-Thymine (AT)-rich DNA. A higher number of hydroxyl (OH) groups caused the TBABr:Gly to form more hydrogen bonds with DNA bases and had the highest ability to quench DAPI from DNA, with Stern-Volmer constants (Ksv) of 115.16 Mâ??1. This study demonstrated that the synthesised DESs were strongly bound to DNA through a combination of electrostatic, hydrophobic, and groove binding. Hence, DES has the potential to solvate and stabilise nucleic acid structures. © 2021 Elsevier B.V.
SN  - 13861425
Y1  - 2021///
TI  - Binding of tetrabutylammonium bromide based deep eutectic solvent to DNA by spectroscopic analysis
KW  - Bromine compounds; DNA; Ethylene; Ethylene glycol; Eutectics; Fluorescence spectroscopy; Hydrogen bonds; Spectroscopic analysis
KW  -  1 
KW  - 3 propanediol (1 
KW  - 3 pd); Binding characteristics; Deep eutectic solvents; Hydrogen bond donors; Interaction mechanisms; Nucleic acid structure; Stern-Volmer constants; Tetrabutylammonium bromide
KW  -  Binding energy
KW  -  DNA; quaternary ammonium derivative; solvent; tetrabutylammonium
KW  -  hydrogen bond
KW  -  DNA; Hydrogen Bonding; Quaternary Ammonium Compounds; Solvents
VL  - 253
PB  - Elsevier B.V.
JF  - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
AV  - none
ER  -