eprintid: 14740 rev_number: 2 eprint_status: archive userid: 1 dir: disk0/00/01/47/40 datestamp: 2023-11-10 03:29:19 lastmod: 2023-11-10 03:29:19 status_changed: 2023-11-10 01:57:41 type: conference_item metadata_visibility: show creators_name: Azhar, A.N.H. creators_name: Anuar, M.A.M. creators_name: Mustapha, S.N.A. creators_name: Amran, N.A. title: Purification of Anthocyanin Extract from Roselle by Progressive Freezing: Effect of coolant temperature and stirring rate ispublished: pub note: cited By 0; Conference of 2021 International Conference on Process Engineering and Advanced Materials, ICPEAM2020 ; Conference Date: 13 July 2021 Through 15 July 2021; Conference Code:185461 abstract: This study presents the process of progressive freezing as a potentially new and alternative approach for the purification of anthocyanin extract to replace the conventional methods of the aqueous two-phase system (ATPS) and adsorption. Specifically, anthocyanins extract was obtained from the roselle plant or also known as red hibiscus. It is essential to purify the anthocyanins extract before it can be used in various applications to remove the by-products of the extract i.e. free sugar, sugar alcohol, organic acids, amino acids, and proteins. Before the purification step, the anthocyanin was first extracted from the roselle flower through a maceration process. The total amount of anthocyanins in the extract was measured by a UV-VIS spectrophotometer. The extract was then concentrated via the progressive freezing process and the influence of cooling temperature and rate of stirring were analysed vis a vis the process performance, which was represented by the effective partition constant (K) and concentration efficiency. The highest concentration efficiency of 55 and the smallest K value of 0.3 were found at the coolant temperature of - 14 °C and 250 rpm rate of stirring. It was also found that lower coolant temperature and higher stirring rate produced a K value that was smaller and superior efficiency. © The Authors, published by EDP Sciences. This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/) date: 2021 publisher: EDP Sciences official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145457118&doi=10.1051%2fe3sconf%2f202128704009&partnerID=40&md5=5975a7112fce8428c0e40760ec55f3d6 id_number: 10.1051/e3sconf/202128704009 full_text_status: none publication: E3S Web of Conferences volume: 287 refereed: TRUE issn: 25550403 citation: Azhar, A.N.H. and Anuar, M.A.M. and Mustapha, S.N.A. and Amran, N.A. (2021) Purification of Anthocyanin Extract from Roselle by Progressive Freezing: Effect of coolant temperature and stirring rate. In: UNSPECIFIED.