eprintid: 14642 rev_number: 2 eprint_status: archive userid: 1 dir: disk0/00/01/46/42 datestamp: 2023-11-10 03:29:14 lastmod: 2023-11-10 03:29:14 status_changed: 2023-11-10 01:57:26 type: article metadata_visibility: show creators_name: Azmi, A.A.B. creators_name: Chew, K.W. creators_name: Chia, W.Y. creators_name: Mubashir, M. creators_name: Sankaran, R. creators_name: Lam, M.K. creators_name: Lim, J.W. creators_name: Ho, Y.-C. creators_name: Show, P.L. title: Green bioprocessing of protein from Chlorella vulgaris microalgae towards circular bioeconomy ispublished: pub keywords: Biomolecules; Extraction; Flotation; Purification; Recovery, Bioprocessing; Chlorella vulgaris; Downstream-processing; Electrical extraction; Micro-algae; Microalgal biomolecule; Processes integrations; Protein recovery; Recovery yield; Three-phase partitioning, Proteins, green alga; microalga; production cost; protein; purification, article; Chlorella vulgaris; downstream processing; extraction; flotation; microalga; nonhuman; phase partitioning; scale up; biomass; electricity, Chlorella vulgaris, Biomass; Chlorella vulgaris; Electricity; Microalgae note: cited By 13 abstract: The work aimed to study the potential in producing a system with high microalgal protein recovery and separation by utilizing a one-step or integrated downstream process. This in turn enables green biorefinery of protein, contributing to circular bioeconomy whereby less energy, labor, and cost are required for the process. By utilizing electric three phase partitioning flotation system, high protein recovery yield, R of 99.42 ± 0.52 and high separation efficiency, E of 52.72 ± 0.40 system was developed. Scaling up also showed high protein recovery yield with R value of 89.13 ± 1.56. Total processing duration (extraction, separation, and purification) was also significantly reduced to 10 min. This system showed remarkable potential in reducing processing time, alternatively cost of production, benefiting microalgal downstream processing. Concisely, through this system, microalgal bioprocessing will no longer be complex allowing a wide array of potentials for further studies in this field. © 2021 Elsevier Ltd date: 2021 publisher: Elsevier Ltd official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105829231&doi=10.1016%2fj.biortech.2021.125197&partnerID=40&md5=0f8875e1a912941c2d157c40b2c1039a id_number: 10.1016/j.biortech.2021.125197 full_text_status: none publication: Bioresource Technology volume: 333 refereed: TRUE issn: 09608524 citation: Azmi, A.A.B. and Chew, K.W. and Chia, W.Y. and Mubashir, M. and Sankaran, R. and Lam, M.K. and Lim, J.W. and Ho, Y.-C. and Show, P.L. (2021) Green bioprocessing of protein from Chlorella vulgaris microalgae towards circular bioeconomy. Bioresource Technology, 333. ISSN 09608524