TY - JOUR KW - Biomolecules; Extraction; Flotation; Purification; Recovery KW - Bioprocessing; Chlorella vulgaris; Downstream-processing; Electrical extraction; Micro-algae; Microalgal biomolecule; Processes integrations; Protein recovery; Recovery yield; Three-phase partitioning KW - Proteins KW - green alga; microalga; production cost; protein; purification KW - article; Chlorella vulgaris; downstream processing; extraction; flotation; microalga; nonhuman; phase partitioning; scale up; biomass; electricity KW - Chlorella vulgaris KW - Biomass; Chlorella vulgaris; Electricity; Microalgae ID - scholars14642 TI - Green bioprocessing of protein from Chlorella vulgaris microalgae towards circular bioeconomy N2 - The work aimed to study the potential in producing a system with high microalgal protein recovery and separation by utilizing a one-step or integrated downstream process. This in turn enables green biorefinery of protein, contributing to circular bioeconomy whereby less energy, labor, and cost are required for the process. By utilizing electric three phase partitioning flotation system, high protein recovery yield, R of 99.42 ± 0.52 and high separation efficiency, E of 52.72 ± 0.40 system was developed. Scaling up also showed high protein recovery yield with R value of 89.13 ± 1.56. Total processing duration (extraction, separation, and purification) was also significantly reduced to 10 min. This system showed remarkable potential in reducing processing time, alternatively cost of production, benefiting microalgal downstream processing. Concisely, through this system, microalgal bioprocessing will no longer be complex allowing a wide array of potentials for further studies in this field. © 2021 Elsevier Ltd N1 - cited By 13 AV - none VL - 333 UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105829231&doi=10.1016%2fj.biortech.2021.125197&partnerID=40&md5=0f8875e1a912941c2d157c40b2c1039a JF - Bioresource Technology A1 - Azmi, A.A.B. A1 - Chew, K.W. A1 - Chia, W.Y. A1 - Mubashir, M. A1 - Sankaran, R. A1 - Lam, M.K. A1 - Lim, J.W. A1 - Ho, Y.-C. A1 - Show, P.L. SN - 09608524 PB - Elsevier Ltd Y1 - 2021/// ER -