@article{scholars14642,
          volume = {333},
            note = {cited By 13},
             doi = {10.1016/j.biortech.2021.125197},
            year = {2021},
           title = {Green bioprocessing of protein from Chlorella vulgaris microalgae towards circular bioeconomy},
         journal = {Bioresource Technology},
       publisher = {Elsevier Ltd},
            issn = {09608524},
          author = {Azmi, A. A. B. and Chew, K. W. and Chia, W. Y. and Mubashir, M. and Sankaran, R. and Lam, M. K. and Lim, J. W. and Ho, Y.-C. and Show, P. L.},
        keywords = {Biomolecules; Extraction; Flotation; Purification; Recovery, Bioprocessing; Chlorella vulgaris; Downstream-processing; Electrical extraction; Micro-algae; Microalgal biomolecule; Processes integrations; Protein recovery; Recovery yield; Three-phase partitioning, Proteins, green alga; microalga; production cost; protein; purification, article; Chlorella vulgaris; downstream processing; extraction; flotation; microalga; nonhuman; phase partitioning; scale up; biomass; electricity, Chlorella vulgaris, Biomass; Chlorella vulgaris; Electricity; Microalgae},
             url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105829231&doi=10.1016\%2fj.biortech.2021.125197&partnerID=40&md5=0f8875e1a912941c2d157c40b2c1039a},
        abstract = {The work aimed to study the potential in producing a system with high microalgal protein recovery and separation by utilizing a one-step or integrated downstream process. This in turn enables green biorefinery of protein, contributing to circular bioeconomy whereby less energy, labor, and cost are required for the process. By utilizing electric three phase partitioning flotation system, high protein recovery yield, R of 99.42 {\^A}{$\pm$} 0.52 and high separation efficiency, E of 52.72 {\^A}{$\pm$} 0.40 system was developed. Scaling up also showed high protein recovery yield with R value of 89.13 {\^A}{$\pm$} 1.56. Total processing duration (extraction, separation, and purification) was also significantly reduced to 10 min. This system showed remarkable potential in reducing processing time, alternatively cost of production, benefiting microalgal downstream processing. Concisely, through this system, microalgal bioprocessing will no longer be complex allowing a wide array of potentials for further studies in this field. {\^A}{\copyright} 2021 Elsevier Ltd}
}