TY - JOUR AV - none SP - 1257 TI - Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization study for biodiesel production N1 - cited By 11 SN - 20950179 PB - Higher Education Press Limited Company EP - 1268 KW - Algae; Biodiesel; Cells; Cytology; Microorganisms; Oleic acid; Ozonization; Palmitic acid KW - Biodiesel production; Cell disruption; Characterization studies; Chlorella vulgaris; Control samples; Lipid extraction; Methyl palmitate; Ozone concentration KW - Ozone ID - scholars14443 N2 - In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells (Chlorella vulgaris) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·hâ??1 ozone concentration, 30 min ozonation time, 1 L·minâ??1 of ozone flowrate at medium pH of 10 and temperature of 30 °C), the sedimentation efficiency of microalgae cells increased significantly from 12.56 to 68.62. It was observed that the microalgae cells aggregated to form flocs after pretreated with ozone due to the increment of surface charge from â??20 to â??6.59 mV. Besides, ozone had successfully disrupted the microalgae cells and resulted in efficient lipid extraction, which was 1.9 times higher than the control sample. The extracted microalgae lipid was mainly consisted of methyl palmitate (C16:0), methyl oleate (C18:1) and methyl linolenate (C18:3), making it suitable for biodiesel production. Finally, utilization of recycled culture media after ozonation pre-treatment showed robust growth of microalgae, in which the biomass yield was maintained in the range of 0.796 to 0.879 g ·hâ??1 for 5 cycles of cultivation. Figure not available: see fulltext.. © 2021, Higher Education Press. IS - 5 Y1 - 2021/// VL - 15 UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100838032&doi=10.1007%2fs11705-020-2015-9&partnerID=40&md5=b16844e3995cd9081fc22ca79c66cfdb A1 - Kadir, W.N.A. A1 - Lam, M.K. A1 - Uemura, Y. A1 - Lim, J.W. A1 - Kiew, P.L. A1 - Lim, S. A1 - Rosli, S.S. A1 - Wong, C.Y. A1 - Show, P.L. A1 - Lee, K.T. JF - Frontiers of Chemical Science and Engineering ER -