eprintid: 14201 rev_number: 2 eprint_status: archive userid: 1 dir: disk0/00/01/42/01 datestamp: 2023-11-10 03:28:46 lastmod: 2023-11-10 03:28:46 status_changed: 2023-11-10 01:56:17 type: article metadata_visibility: show creators_name: Tong, K.T.X. creators_name: Tan, I.S. creators_name: Foo, H.C.Y. creators_name: Tiong, A.C.Y. creators_name: Lam, M.K. creators_name: Lee, K.T. title: Third-generation L-Lactic acid production by the microwave-assisted hydrolysis of red macroalgae Eucheuma denticulatum extract ispublished: pub keywords: Bacteriology; Fermentation; Hydrolysis; Lactic acid, Carrageenans; Chlorella vulgaris; Chlorellum vulgari residue; High density fermentations; Hydrothermal hydrolysis; Lactobacillus acidophilus; Lactobacillus acidophilus LA-14; Macro-algae; Red macroalga carrageenan; Third generation, Bacilli, algal extract; Eucheuma denticulatum extract; galactose; lactic acid; unclassified drug; lactic acid; plant extract, chemical compound; detection method; fermentation; hydrolysis; plant extract; red alga; temperature effect, acid hydrolysis; Article; Bacillus coagulans; cell density; chemical composition; Chlorella vulgaris; Eucheuma; Eucheuma denticulatum; fermentation; fractionation; inoculation; Lactobacillus acidophilus; microwave assisted extraction; nonhuman; nutrient; process optimization; supplementation; Chlorella vulgaris; hydrolysis; microwave radiation; seaweed, Bacillus coagulans; Chlorella vulgaris; Eucheuma denticulatum; Lactobacillus acidophilus, Chlorella vulgaris; Fermentation; Hydrolysis; Lactic Acid; Microwaves; Plant Extracts; Seaweed note: cited By 13 abstract: The development of an efficient third-generation L-lactic acid (L-LA) production process from Eucheuma denticulatum extract (EDE) was achieved in this study. Microwave-assisted dilute acid hydrolysis (MADAH) and microwave-assisted hydrothermal hydrolysis (MAHTH) were chosen as the hydrolysis of EDE for the objective of increasing galactose yield. Single-factor optimization of hydrolysis of the EDE was studied, MADAH had high performance in galactose production relative to MAHTH, in which the yield and optimal conditions for both processes were 50.7 (0.1 M H2SO4, 120 °C for 25 min) and 47.8 (0 M H2SO4,160 °C for 35 min), respectively. For fermentation, the optimal L-LA yield was achieved at the inoculum cell density of 4 (w/w) Bacillus coagulans ATCC 7050 with 89.4 and 6 (w/w) Lactobacillus acidophilus LA-14 with 87.6. In addition, lipid-extracted Chlorella vulgaris residues (CVRs) as co-nutrient supplementation increased the relative abundance of B. coagulans ATCC 7050, thus benefiting L-LA production. © 2021 Elsevier Ltd date: 2021 publisher: Elsevier Ltd official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116067938&doi=10.1016%2fj.biortech.2021.125880&partnerID=40&md5=ab9ea6927a2ee0209e2247f95146a753 id_number: 10.1016/j.biortech.2021.125880 full_text_status: none publication: Bioresource Technology volume: 342 refereed: TRUE issn: 09608524 citation: Tong, K.T.X. and Tan, I.S. and Foo, H.C.Y. and Tiong, A.C.Y. and Lam, M.K. and Lee, K.T. (2021) Third-generation L-Lactic acid production by the microwave-assisted hydrolysis of red macroalgae Eucheuma denticulatum extract. Bioresource Technology, 342. ISSN 09608524