eprintid: 14189
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/01/41/89
datestamp: 2023-11-10 03:28:45
lastmod: 2023-11-10 03:28:45
status_changed: 2023-11-10 01:56:15
type: article
metadata_visibility: show
creators_name: Subramani, I.G.
creators_name: Perumal, V.
creators_name: Gopinath, S.C.B.
creators_name: Mohamed, N.M.
creators_name: Ovinis, M.
creators_name: Sze, L.L.
title: 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
ispublished: pub
keywords: allergen; aptamer; copper; graphite; imidazole derivative; lactoglobulin; N,N-carbonyldiimidazole; nanomaterial, animal; chemistry; electrochemical analysis; food analysis; genetic procedures; limit of detection; milk; procedures, Allergens; Animals; Aptamers, Nucleotide; Biosensing Techniques; Copper; Electrochemical Techniques; Food Analysis; Graphite; Imidazoles; Lactoglobulins; Limit of Detection; Milk; Nanostructures
note: cited By 6
abstract: The bovine milk allergenic protein, �β-lactoglobulin� is one of the leading causes of milk allergic reaction. In this research, a novel label-free non-faradaic capacitive aptasensor was designed to detect β-lactoglobulin using a Laser Scribed Graphene (LSG) electrode. The graphene was directly engraved into a microgapped (~ 95 µm) capacitor-electrode pattern on a flexible polyimide (PI) film via a simple one-step CO2 laser irradiation. The novel hybrid nanoflower (NF) was synthesized using 1,1�-carbonyldiimidazole (CDI) as the organic molecule and copper (Cu) as the inorganic molecule via one-pot biomineralization by tuning the reaction time and concentration. NF was fixed on the pre-modified PI film at the triangular junction of the LSG microgap specifically for bio-capturing β-lactoglobulin. The fine-tuned CDI-Cu NF revealed the flower-like structures was viewed through field emission scanning electron microscopy. Fourier-transform infrared spectroscopy showed the interactions with PI film, CDI-Cu NF, oligoaptamer and β-lactoglobulin. The non-faradaic sensing of milk allergen β-lactoglobulin corresponds to a higher loading of oligoaptamer on 3D-structured CDI-Cu NF, with a linear range detection from 1 ag/ml to 100 fg/ml and attomolar (1 ag/ml) detection limit (S/N = 3:1). This novel CDI-Cu NF/LSG microgap aptasensor has a great potential for the detection of milk allergen with high-specificity and sensitivity. © 2021, The Author(s).
date: 2021
publisher: Nature Research
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117693457&doi=10.1038%2fs41598-021-00057-4&partnerID=40&md5=760e8935ae099cadaea9e718bda6aff1
id_number: 10.1038/s41598-021-00057-4
full_text_status: none
publication: Scientific Reports
volume: 11
number: 1
refereed: TRUE
issn: 20452322
citation:   Subramani, I.G. and Perumal, V. and Gopinath, S.C.B. and Mohamed, N.M. and Ovinis, M. and Sze, L.L.  (2021) 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen.  Scientific Reports, 11 (1).   ISSN 20452322