TY  - JOUR
Y1  - 2021///
SN  - 20452322
PB  - Nature Research
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117693457&doi=10.1038%2fs41598-021-00057-4&partnerID=40&md5=760e8935ae099cadaea9e718bda6aff1
A1  - Subramani, I.G.
A1  - Perumal, V.
A1  - Gopinath, S.C.B.
A1  - Mohamed, N.M.
A1  - Ovinis, M.
A1  - Sze, L.L.
JF  - Scientific Reports
VL  - 11
AV  - none
IS  - 1
N2  - The bovine milk allergenic protein, â??β-lactoglobulinâ?? is one of the leading causes of milk allergic reaction. In this research, a novel label-free non-faradaic capacitive aptasensor was designed to detect β-lactoglobulin using a Laser Scribed Graphene (LSG) electrode. The graphene was directly engraved into a microgapped (~ 95 µm) capacitor-electrode pattern on a flexible polyimide (PI) film via a simple one-step CO2 laser irradiation. The novel hybrid nanoflower (NF) was synthesized using 1,1â?²-carbonyldiimidazole (CDI) as the organic molecule and copper (Cu) as the inorganic molecule via one-pot biomineralization by tuning the reaction time and concentration. NF was fixed on the pre-modified PI film at the triangular junction of the LSG microgap specifically for bio-capturing β-lactoglobulin. The fine-tuned CDI-Cu NF revealed the flower-like structures was viewed through field emission scanning electron microscopy. Fourier-transform infrared spectroscopy showed the interactions with PI film, CDI-Cu NF, oligoaptamer and β-lactoglobulin. The non-faradaic sensing of milk allergen β-lactoglobulin corresponds to a higher loading of oligoaptamer on 3D-structured CDI-Cu NF, with a linear range detection from 1 ag/ml to 100 fg/ml and attomolar (1 ag/ml) detection limit (S/N = 3:1). This novel CDI-Cu NF/LSG microgap aptasensor has a great potential for the detection of milk allergen with high-specificity and sensitivity. © 2021, The Author(s).
N1  - cited By 6
KW  - allergen; aptamer; copper; graphite; imidazole derivative; lactoglobulin; N
KW  - N-carbonyldiimidazole; nanomaterial
KW  -  animal; chemistry; electrochemical analysis; food analysis; genetic procedures; limit of detection; milk; procedures
KW  -  Allergens; Animals; Aptamers
KW  -  Nucleotide; Biosensing Techniques; Copper; Electrochemical Techniques; Food Analysis; Graphite; Imidazoles; Lactoglobulins; Limit of Detection; Milk; Nanostructures
ID  - scholars14189
TI  - 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
ER  -