@article{scholars14189,
            year = {2021},
       publisher = {Nature Research},
         journal = {Scientific Reports},
             doi = {10.1038/s41598-021-00057-4},
            note = {cited By 6},
          volume = {11},
          number = {1},
           title = {1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen},
        keywords = {allergen; aptamer; copper; graphite; imidazole derivative; lactoglobulin; N,N-carbonyldiimidazole; nanomaterial, animal; chemistry; electrochemical analysis; food analysis; genetic procedures; limit of detection; milk; procedures, Allergens; Animals; Aptamers, Nucleotide; Biosensing Techniques; Copper; Electrochemical Techniques; Food Analysis; Graphite; Imidazoles; Lactoglobulins; Limit of Detection; Milk; Nanostructures},
             url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117693457&doi=10.1038\%2fs41598-021-00057-4&partnerID=40&md5=760e8935ae099cadaea9e718bda6aff1},
        abstract = {The bovine milk allergenic protein, {\^a}??{\^I}2-lactoglobulin{\^a}?? is one of the leading causes of milk allergic reaction. In this research, a novel label-free non-faradaic capacitive aptasensor was designed to detect {\^I}2-lactoglobulin using a Laser Scribed Graphene (LSG) electrode. The graphene was directly engraved into a microgapped ({\texttt{\char126}} 95{\^A} {\^A}um) capacitor-electrode pattern on a flexible polyimide (PI) film via a simple one-step CO2 laser irradiation. The novel hybrid nanoflower (NF) was synthesized using 1,1{\^a}?2-carbonyldiimidazole (CDI) as the organic molecule and copper (Cu) as the inorganic molecule via one-pot biomineralization by tuning the reaction time and concentration. NF was fixed on the pre-modified PI film at the triangular junction of the LSG microgap specifically for bio-capturing {\^I}2-lactoglobulin. The fine-tuned CDI-Cu NF revealed the flower-like structures was viewed through field emission scanning electron microscopy. Fourier-transform infrared spectroscopy showed the interactions with PI film, CDI-Cu NF, oligoaptamer and {\^I}2-lactoglobulin. The non-faradaic sensing of milk allergen {\^I}2-lactoglobulin corresponds to a higher loading of oligoaptamer on 3D-structured CDI-Cu NF, with a linear range detection from 1{\^A} ag/ml to 100{\^A} fg/ml and attomolar (1 ag/ml) detection limit (S/N = 3:1). This novel CDI-Cu NF/LSG microgap aptasensor has a great potential for the detection of milk allergen with high-specificity and sensitivity. {\^A}{\copyright} 2021, The Author(s).},
            issn = {20452322},
          author = {Subramani, I. G. and Perumal, V. and Gopinath, S. C. B. and Mohamed, N. M. and Ovinis, M. and Sze, L. L.}
}