eprintid: 13203 rev_number: 2 eprint_status: archive userid: 1 dir: disk0/00/01/32/03 datestamp: 2023-11-10 03:27:46 lastmod: 2023-11-10 03:27:46 status_changed: 2023-11-10 01:50:35 type: article metadata_visibility: show creators_name: Koyande, A.K. creators_name: Chew, K.W. creators_name: Lim, J.-W. creators_name: Lam, M.-K. creators_name: Ho, Y.-C. creators_name: Show, P.-L. title: Biorefinery of Chlorella sorokiniana using ultra sonication assisted liquid triphasic flotation system ispublished: pub keywords: Algae; Flotation; Liquids; Microorganisms; Proteins; Refining; Sulfur compounds, Ammonium sulphate concentration; Biorefineries; Chlorella sorokiniana; Lipid recoveries; Micro-algae; Protein recovery; Tri-Phasic; Ultra-sonication, Bioconversion, alcohol; ammonium sulfate; lipid, alcohol; ammonium sulfate; biomass; concentration (composition); extraction method; microalga; protein; recycling; ultrasonics, Article; biomass; Chlorella sorokiniana; concentration (parameter); flotation; flow rate; microalga; nonhuman; priority journal; protein analysis; protein isolation; ultrasound; Chlorella; microalga; recycling; ultrasound, Chlorella sorokiniana, Biomass; Chlorella; Microalgae; Recycling; Sonication note: cited By 18 abstract: The aim of this work was to study the ultrasonication-assisted Liquid Tri-phasic Flotation (LTF) System to obtain lipid and protein from microalgae Chlorella sorokiniana in a single step as a novel process. In the current study, biorefinery of Chlorella sorokiniana was performed using LTF system in a single step. The highest protein recovery of 97.43 ± 1.67 and lipid recovery of 69.50 ± 0.54 were obtained. The corresponding parameters were microalgae biomass loading of 0.5 w/v, ammonium sulphate concentration of 40 w/v, volume ratio of 1:1.5 (salt:alcohol), ultrasonication pulse mode of 20 s ON/20 s OFF at 20 amplitude for 5 mins, flotation air flowrate of 100 mL/min. Additionally, recycling of alcohol phase to study the circular nature of proposed biorefinery was investigated. The proposed LTF system for extraction of proteins and lipid reduces the number of operation units required in this biorefinery approach. © 2020 Elsevier Ltd date: 2020 publisher: Elsevier Ltd official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078999051&doi=10.1016%2fj.biortech.2020.122931&partnerID=40&md5=995632c41eaf34b9ce5fcc1ea0b708a4 id_number: 10.1016/j.biortech.2020.122931 full_text_status: none publication: Bioresource Technology volume: 303 refereed: TRUE issn: 09608524 citation: Koyande, A.K. and Chew, K.W. and Lim, J.-W. and Lam, M.-K. and Ho, Y.-C. and Show, P.-L. (2020) Biorefinery of Chlorella sorokiniana using ultra sonication assisted liquid triphasic flotation system. Bioresource Technology, 303. ISSN 09608524